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为了探讨甘草黄酮对长期大强度运动小鼠心肌损伤的保护作用机制。本研究选用50只昆明雄性小鼠为研究对象,以每组10只分组,分为安静对照组(A组)、运动训练组(B组)、运动低剂量给药组(C组)、运动中剂量给药组(D组)、以及运动高剂量给药组(E组)。运动低剂量给药组(C组)、运动中剂量给药组(D组)、运动高剂量给药组(E组)训练前分别灌服剂量为5 g/kg/d、10 g/kg/d、15 g/kg/d黄酮溶液;除安静对照组外,运动组小鼠进行为期6周的大强度游泳训练。测定小鼠各组小鼠血清CK-MB、c Tn I,心肌组织中GSH-PX、SOD、CAT、MDA、NOS、NO、Na+/K+-ATP、Ca2+/Mg2+-ATP。结果表明:与安静对照组比较,运动训练组CK-MB、c Tn I、MDA、NO、NOS升高,GSH-PX、SOD、CAT、Na+/K+-ATP、Ca2+/Mg2+-ATP降低。与运动训练组比较,运动低剂量给药组、运动中剂量给药组、运动高剂量给药组CK-MB、c Tn I、MDA、NO、NOS降低,GSH-PX、SOD、CAT、Na+/K+-ATP、Ca2+/Mg2+-ATP升高;与运动低剂量给药组比较,运动高剂量给药组CK-MB、c Tn I、GSH-Px、SOD、Na+/K+-ATP、Ca2+/Mg2+-ATP升高,MDA降低。本研究提示,长期大强度运动导致机体心肌损伤,甘草黄酮可提高机体心肌组织中抗氧酶、ATPase酶的活性,抑制自由基生成、NOS活性,减少NO的生成,维持细胞膜内外Na+、K+、Ca2+、Mg2+的分布平衡,对大强度运动造成的心肌损伤具有保护作用,效果以高剂量黄酮最佳。
In order to explore the protective effect of licorice flavonoids on myocardial injury in long-term high-intensity exercise mice. In this study, 50 Kunming male mice were selected as research objects, and divided into 10 groups: control group (group A), exercise group (group B), exercise group (group C) Middle dose group (group D) and high dose exercise group (group E). The rats in low-dose exercise group (C group), middle-dose exercise group (D group) and high-exercise exercise group (E group) were given 5 g / kg / d and 10 g / kg / d, 15 g / kg / d flavonoid solution; except for the quiet control group, exercise group of mice for 6 weeks of intensive swimming training. Serum CK-MB, cTn I, GSH-PX, SOD, CAT, MDA, NOS, NO, Na + / K + -ATP and Ca2 + / Mg2 + -ATP were measured in mice. The results showed that compared with the control group, the levels of CK-MB, cTn I, MDA, NO and NOS increased and the levels of GSH-PX, SOD, CAT, Na + / K + -ATP and Ca2 + / Mg2 + -ATP decreased in the exercise training group. Compared with exercise training group, the levels of CK-MB, cTn I, MDA, NO, NOS, GSH-PX, SOD, CAT and Na + in exercise low dose group, exercise dose group and exercise high dose group decreased The levels of CK-MB, cTn I, GSH-Px, SOD, Na + / K + -ATP and Ca2 + / ATPase in the high-dose exercise group were significantly higher than those in the low- Mg2 + -ATP increased MDA decreased. This study suggests that long-term high-intensity exercise can lead to myocardial damage, and that glycyrrhiza flavonoids can increase the activities of antioxidant enzyme and ATPase in myocardium, inhibit the production of free radicals and NOS activity, decrease the production of NO and maintain the contents of Na +, K + Ca2 +, Mg2 + balance of the distribution of the myocardial injury caused by intensive exercise have a protective effect, the effect of high-dose flavonoids best.