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目的:建立以高效液相色谱法同时测定石韦配方颗粒中绿原酸、咖啡酸及芒果苷含量的方法。方法:色谱柱为Shim-pack VP-ODS柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.1%磷酸(9∶91);检测波长0～30 min为323 nm,30.1～45min为258 nm;流速为1.0 mL·min-1。结果:绿原酸在129.6～302.4 mg·L-1(r=0.999 8)线性关系良好,平均回收率为98.15%,RSD 1.69%;咖啡酸在13.8～32.2 mg·L-1(r=0.999 9)线性关系良好,平均回收率为99.93%,RSD 2.98%;芒果苷在151.2～352.8 mg·L-1(r=0.999 9)线性关系良好,平均回收率为101.43%,RSD 2.00%。结论:本法简便、快速、重复性好,可用于石韦配方颗粒的质量控制。
Objective: To establish a method for simultaneous determination of chlorogenic acid, caffeic acid and mangiferin in Shiwei formula granules by high performance liquid chromatography. METHODS: The chromatographic column was Shim-pack VP-ODS column (4.6 mm × 250 mm, 5 μm). The mobile phase was acetonitrile-0.1% phosphoric acid (9:91). The detection wavelength ranged from 30 min to 323 nm. 258 nm; flow rate 1.0 mL · min-1. Results: The linearity of chlorogenic acid was good at 129.6 ~ 302.4 mg · L-1 (r = 0.999 8) with an average recovery of 98.15% and a RSD of 1.69%. Caffeic acid was at 13.8 ~ 32.2 mg · L-1 9). The average recovery was 99.93% and the RSD was 2.98%. The linear relationship of mangiferin was 151.2 ~ 352.8 mg · L-1 (r = 0.999 9). The average recovery was 101.43% and RSD was 2.00%. Conclusion: This method is simple, rapid, reproducible, and can be used for the quality control of Shiwei formula particles.