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目的了解郧县禽流感高危职业人群H5N1感染及外环境禽流感病毒H5、H9亚型污染状况。方法 2011年1-3月采集从事家禽养殖、屠宰等100人血清标本用红细胞凝集抑制试验(HI)检测H5N1抗体;1、4、7、10月每月各采集10份外环境鸡笼、鸡粪、污水标本用荧光定量PCR法(Real-time PCR)检测禽流感病毒FluA、AH5、AH9、AN1核酸。结果 100份高危职业人群血清H5N1抗体均为阴性;家禽养殖户11份外环境标本FluA、AH5、AH9、AN1核酸均为阴性;家禽屠宰点29份外环境标本中,阳性检出(率)分别为FluA 10份(34.48%)、AH5 8份(27.59%)、AH9阳性4份(13.79%)、AN1 1份(3.45%),1份污水标本中AH5、AN1阳性。结论郧县家禽屠宰点外环境存在高致病性禽流感病毒(H5N1)污染,家禽屠宰点为人感染禽流感的高风险场所。
Objective To understand the H5N1 infection in high-risk occupational groups of bird flu in Yun County and the contamination status of H5 and H9 in the external environment of bird flu virus. Methods From January to March in 2011, 100 human serum samples from poultry breeding and slaughtering were collected and tested for H5N1 antibody by hemagglutination inhibition (HI) assay. In January, April, July and October, 10 samples of external environment cages and chicken Dung and sewage samples Fluorescence quantitative PCR (Real-time PCR) was used to detect FluA, AH5, AH9 and AN1 nucleic acids of avian influenza virus. Results Serum H5N1 antibody was negative in 100 high-risk occupational groups; 11 samples of poultry farmer’s external environment were negative for FluA, AH5, AH9 and AN1; in 29 samples of poultry slaughter area, the positive detection rate was AHA and AN1 were positive for 1 part of FluA (34.48%), AH5 (27.59%), AH9 positive (13.79%), AN1 1 part (3.45%) and 1 part of sewage samples. CONCLUSION: H5N1 contamination exists in the external environment of poultry slaughterhouses in Yun County, and the poultry slaughter point is a high-risk site for people infected with bird flu.