目的比较ｆａｓ基因和ｂｃｌ２基因在胃癌耐药细胞与非耐药细胞表达的差异，将ｆａｓ基因和ｂｃｌ２反义核酸导入胃癌耐药细胞，并分析转导前后的细胞在ｍＲＮＡ与蛋白的表达水平，研究转导株与非转导株对化疗药物敏感性的区别．方法采用分子克隆技术将ｆａｓ基因和反义ｂｃｌ２片段分别插入真核表达载体ｐＢＫＣＭＶ和ｐＤＯＲＳＶ４０的多克隆克隆位点之间，以脂质体介导法将两个重组表达载体分别转染受体细胞ＳＧＣ７９０１／ＶＣＲ，Ｇ４１８筛选克隆细胞，Ｎｏｒｔｈｅｒｎｂｌｏｔ，Ｗｅｓｔｅｒｎｂｌｏｔ检测耐药细胞与非耐药细胞以及转导细胞中ｆａｓ基因和ｂｃｌ２基因ｍＲＮＡ及其蛋白的表达，ＭＴＴ法药敏实验检测转导细胞与非转导细胞对ＶＣＲ、顺铂、５ＦＵ的敏感性．结果真核表达载体ｐＢＫｆａｓｃＤＮＡ和ｐＤＯＲｂｃｌ２ｃＤＮＡ转导胃癌耐药细胞后，分别从２×１０５细胞中筛选出大约８０和１２０个抗性克隆，转导率各为０４‰和０６‰，随机各挑选２个克隆继续筛选与扩增培养，均获得了稳定的抗性细胞，我们将此命名为ＳＧＣ７９０１ｆａｓ／ＶＣＲｃｅｌ和ＳＧＣ７９０１ａｎｔｉｂｃｌ２／ＶＣＲｃｅｌ．杂交结果表明，胃癌耐药细胞ＳＧＣ７９０１／? Objective To compare the expression of fas gene and bcl2 gene in gastric cancer resistant and non-drug resistant cells, and to introduce fas gene and bcl2 antisense nucleic acid into gastric cancer resistant cells, and analyze the mRNA and protein before and after transduction. The expression level, the difference between the sensitivity of chemotherapeutic drugs in transduced and non-transduced strains. Methods The molecular cloning technique was used to insert the fas gene and antisense bcl2 fragments into the cloning sites of the eukaryotic expression vectors pBKCMV and pDORSV40, respectively. The two recombinant expression vectors were mediated by liposome. Recipient cells were transfected with SGC7901/VCR respectively. G418 was used to screen clonal cells. Northern blot and Western blot were used to detect the expression of fas and bcl2 mRNA and protein in drug-resistant and non-drug resistant cells and transduced cells. MTT assay drug sensitivity The sensitivity of VCR, cisplatin and 5FU in transduced cells and non-transduced cells was tested. RESULTS: Eukaryotic expression vectors pBK-fascDNA and pDOR-bcl-2 cDNA were used to transduce gastric cancer drug-resistant cells. About 80 and 120 resistant clones were selected from 2×105 cells respectively. The transduction rates were 04‰ and For 06‰, two randomly selected clones were selected for further screening and expansion, and stable resistant cells were obtained. We named this as SGC7901fas/VCRcel and SGC7901antibcl2/VCRcel. The hybridization results showed that gastric cancer resistant cells SGC7901/?