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目的建立人血管瘤裸小鼠异种移植模型并动态观察移植物形态学变化。方法将人婴幼儿血管瘤组织切分为96个小块移植于16只裸小鼠皮下,定期测量瘤体体积,取不同时期移植瘤作HE染色和抗人CD31免疫荧光标记。结果移植瘤大部分(81/96)存活,在移植后1～2个月期间体积明显增大。继而缓慢缩小。组织学观察显示移植瘤早期出现缺血改变,瘤性内皮细胞坏死、间质纤维化,约在移植后1个月出现瘤细胞增生,血管瘤增长,继而瘤内血管扩张,瘤性内皮细胞逐渐减少,血管瘤消退,代以纤维脂肪组织。结论将快速生长的人血管瘤组织移植于裸小鼠体内,移植瘤可存活,其缺血期后生长模式与人血管瘤自然发展过程相似。
Objective To establish a xenograft model of human hemangioma in nude mice and dynamically observe the morphological changes of the graft. Methods Human infantile hemangiomas were divided into 96 small sections and subcutaneously transplanted into 16 nude mice. The volume of the tumor was measured regularly. HE staining and anti-human CD31 immunofluorescence staining were performed on the transplanted tumor at different stages. Results Most of the xenografts (81/96) survived, with a significant increase in volume from 1 to 2 months after transplantation. Then slowly narrowing. Histological observation showed that the early xenograft tumors had ischemic changes, tumorous endothelial cell necrosis and interstitial fibrosis. About one month after transplantation, tumor cells proliferated, hemangiomas grew, and then tumor vessels expanded and tumorous endothelial cells gradually developed Reduce, hemangioma subsided, on behalf of the fibrous adipose tissue. Conclusion The rapid growth of human hemangioma tissue transplanted in nude mice, transplanted tumor can survive, its growth pattern after ischemia and human hemangioma similar to the natural development process.