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目的 :由于具有强的致龋潜力耐氟菌株的产生 ,氟化物的单一应用存在一定的局限性。本组观察含氟的微量元素矿化液对变链菌生长和粘附的影响。方法 :采用厌氧菌连续培养技术 ,用菌液的吸光度(ABS)和菌落计数作指标 ,描述矿化液对变链菌生长繁殖的影响 ;用3H -胸腺嘧啶核苷 (3H -TdR)标记变链菌的方法 ,检测矿化液对变链菌粘附的影响。结果 :氟矿化液及氟微量元素矿化液均具有抑制细菌生长的作用 ,同时能抑制变链菌对S -HA表面的粘附 ,以氟微量元素矿化液的作用较强。结论 :氟微量元素矿化液比氟矿化液具有更好的抑制变链菌生长及对S -HA表面的粘附 ,提示氟与微量元素联合应用 ,在减少氟用量的同时具有更好的防龋效果
OBJECTIVE: The single use of fluoride has its own limitations due to the fluoride-resistant strains that have strong cariogenic potential. This group observed fluoride-containing trace mineralization of Streptococcus mutans growth and adhesion. Methods: Continuous culture of anaerobic bacteria was used to describe the effects of mineralized liquid on the growth and reproduction of streptococcus mutans by using the absorbance (ABS) of bacterial liquid and the colony count as indexes. The 3H-thymidine (3H-TdR) Streptococcus mutans method to detect the mineralization of Streptococcus mutans adhesion. Results: Fluorine mineralization fluid and fluorine trace element mineralized fluid both inhibited the growth of bacteria and inhibited the adhesion of S. mutans to S-HA surface. CONCLUSIONS: Fluoride and trace element mineralized fluid has better inhibition of S. mutans growth and adhesion to S-HA surface than fluorinated mineralized fluid, suggesting the combination of fluorine and trace elements has the better effect of reducing fluoride content Anti-caries effect