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目的:探讨邻苯二酸二丁酯(DBP)、己烯雌酚(DES)、氟他胺(Flu)宫内诱导SD鼠隐睾对生殖母细胞(Go)发育的影响。方法:SD孕鼠于妊娠12~21 d每日给予Flu(25 mg/kg,n=15)、DES(1.5μg/kg,n=15)皮下注射,DBP(500 mg/kg,n=10)灌胃,仔鼠出生后第1、10、20、80日取仔鼠睾丸组织,通过免疫组织化学及RT-PCR检测神经母细胞黏附分子(neural cell adhesion molecule,NCAM)在睾丸中的表达差异。结果:DES、DBP诱导大鼠曲细精管NCAM表达与玉米油溶剂对照(n=6)和空白对照组(n=6)一致,出生后第1日(PD1)曲细精管管腔内生殖母细胞(Go)位于管腔中央且均呈阳性表达;PD10 Go迁移至基底膜,NCAM表达明显减弱至消失;PD20、PD80隐睾及下降睾丸曲细精管内NCAM阴性表达。Flu诱导PD10及PD20、PD80隐睾组织中可见位于管腔中央的Go残留,NCAM呈阳性表达;RT-PCR结果显示Flu诱导PD10仔鼠睾丸及PD20隐睾的NCAM mRNA表达显著高于2个对照组(P<0.05)。结论:仅Flu诱导的隐睾组织在各时间段均可见Go残留,且NCAM mRNA及蛋白质表达显著高于正常组,该模型更适宜于研究隐睾Go发育缺陷。
Objective: To investigate the effects of dibutyl phthalate (DBP), diethylstilbestrol (DES) and flutamide on the development of germ cells in SD rat cryptorchidism. Methods: Pregnant SD rats were injected subcutaneously with Flu (25 mg / kg, n = 15) and DES (1.5 μg / kg, n = ) Were intragastrically injected. The offspring testis tissues were taken on the 1st, 10th, 20th and 80th day after birth, and the expression of neural cell adhesion molecule (NCAM) in testis was detected by immunohistochemistry and RT-PCR difference. Results: The NCAM expression in rat seminiferous tubules induced by DBP and DBP was consistent with that of corn oil solvent control (n = 6) and blank control group (n = 6) Go was located in the middle of the lumen with positive expression. PD10 Go migrated to the basement membrane, and the expression of NCAM was significantly weakened and disappeared. The expression of NCAM in cryptorchidism of PD20 and PD80 and decreased in testicular seminiferous tubules were negative. Flu-induced PD10 and PD20, PD80 cryptorchidism can be seen in the central cavity of the Go residue, NCAM positive expression; RT-PCR results showed that Flu-induced PD10 pups testis and PD20 cryptorchid NCAM mRNA expression was significantly higher than the two controls Group (P <0.05). Conclusion: Only Flu-induced cryptorchidism can be observed in each period of Go residue, and NCAM mRNA and protein expression was significantly higher than the normal group, the model is more suitable for the study of Go defects in development of cryptorchidism.