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目的:检测鼻息肉组织及正常下鼻甲黏膜腭肺鼻上皮克隆(PLUNC)、Toll样受体2(TLR2)、核转录因子-κB(NF-κB)的表达情况,分析三者表达的相关性,探讨其在鼻息肉发病机制中的作用及临床意义,为鼻息肉的治疗提供理论依据和新的思路。方法:收集2013-06-2014-12期间郑州大学第一附属医院住院的患者标本。以慢性鼻-鼻窦炎伴鼻息肉患者的息肉组织为实验组(46例),以鼻中隔偏曲矫正术患者的正常下鼻甲黏膜为对照组(19例)。苏木精-伊红染色法测定实验组和对照组嗜酸粒细胞(EOS)浸润情况,免疫组织化学技术测定PLUNC、TLR2及NF-κBp65在实验组及对照组中的表达及其分布,探讨PLUNC、TLR2及NF-κBp65在实验组和对照组中的阳性表达情况及其相关性。结果:实验组EOS浸润程度明显高于对照组(χ~2=12.01,P<0.05)。实验组PLUNC的表达明显低于对照组(χ2=16.09,P<0.05),实验组TLR2、NF-κBp65表达明显高于对照组(χ~2=23.74、χ~2=18.59,P<0.05)。相关性分析显示,实验组PLUNC与TLR2、NF-κBp65呈负相关(r=-0.675、r=-0.550,P<0.05),TLR2与NF-κBp65呈正相关(r=0.540,P<0.05)。EOS浸润程度与TLR2及NF-κBp65呈正相关(r=0.417、r=0.470,P<0.05),与PLUNC呈负相关(r=-0.859,P<0.05)。结论:鼻息肉较正常下鼻甲黏膜组织中PLUNC明显降低,TLR2及NF-κB表达明显升高,提示天然免疫功能的下降,感染性因素的加剧,可能与鼻息肉的发病机制有关。
OBJECTIVE: To detect the expression of PLUNC, TLR2 and NF-κB in nasal polyps and normal inferior turbinate mucosa, and to analyze the correlation between them , Explore its role in the pathogenesis of nasal polyps and its clinical significance for the treatment of nasal polyps provide a theoretical basis and new ideas. Methods: The specimens of patients hospitalized in the First Affiliated Hospital of Zhengzhou University from 2013-06-2014-12 were collected. The polyps of patients with chronic rhinosinusitis and nasal polyps were selected as the experimental group (n = 46), and the normal inferior turbinate mucosa of patients with nasal septum deviation correction as the control group (n = 19). The infiltration of eosinophils (EOS) in the experimental and control groups was detected by hematoxylin-eosin staining. The expression and distribution of PLUNC, TLR2 and NF-κBp65 in the experimental and control groups were detected by immunohistochemistry PLUNC, TLR2 and NF-κBp65 in the experimental group and control group, the positive expression and its correlation. Results: The infiltration of EOS in the experimental group was significantly higher than that in the control group (χ ~ 2 = 12.01, P <0.05). The expression of PLUNC in experimental group was significantly lower than that in control group (χ2 = 16.09, P <0.05). The expression of TLR2 and NF-κBp65 in experimental group was significantly higher than that in control group (χ ~ 2 = 23.74, . Correlation analysis showed that there was a negative correlation between PLUNC and TLR2 and NF-κBp65 in the experimental group (r = -0.675, r = -0.550, P <0.05). TLR2 was positively correlated with NF-κBp65 (r = 0.540, P <0.05). EOS infiltration was positively correlated with TLR2 and NF-κBp65 (r = 0.417, r = 0.470, P <0.05), but negatively correlated with PLUNC (r = -0.859, P <0.05). CONCLUSIONS: Compared with normal inferior turbinate mucosa, nasal polyps significantly decreased PLUNC and markedly increased the expression of TLR2 and NF-κB, which suggested that the decrease of innate immune function and the aggravation of infectious factors may be related to the pathogenesis of nasal polyps.