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我们于一九七八年晚季正式开始水稻花粉单倍体育种试验,一九七九年春获得了一批花培种子.参试材料采用优势强、综合性状好的杂种一代和低代的27个组合,其中常规杂交组合19个,籼粳杂交组合2个,杂优组合6个.诱导培养基采用N_6、合五,分化培养基用N_6和MS.为节省配制培养基时间,避免手续过程对所用的各种元素的误差,采取预先配制混合母液贮存于冰箱.培养基一律在1.2公斤/厘米~2压力下灭菌20分钟,调整PH5.8～6.0.以单核靠这期作为接种花药适期先进行镜检核对,在晴夭露水干后采下稻穗装入塑料袋,存放于9℃左右的冰箱内备用.花药接种在接种箱或接种室等严格的无菌操作条件下进行.接种
We formally started the rice pollen haploid breeding test in the late fall of 1978 and obtained a number of flower-seeded seeds in the spring of 1979. The tested materials used strong and well-behaved hybrids and low-generation hybrids 19 combinations, 2 indica-japonica hybrids and 6 hybrid combinations were used as induction medium, N6 and MS were used as induction medium, and N6 and MS were used as differentiation medium in order to save the time of preparation medium and avoid the procedure The error of the various elements used to take a mixture of pre-mixed mother liquor stored in the refrigerator medium were all 1.2 kg / cm ~ 2 pressure sterilization 20 minutes, adjust PH5.8 ~ 6.0 to single-core by this period as vaccination The anther is suitable for the first microscopic examination and collation, and the panicles are collected in a plastic bag and stored in a freezer at about 9 ° C. After anther inoculated in vaccination box or inoculation room and other strict aseptic operating conditions Inoculation