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目的:研究DHA对糖尿病神经病理性痛大鼠机械痛敏和热痛敏的作用,探讨DHA对糖尿病大鼠背根节JNK磷酸化和CXCL1水平及其受体表达的影响。方法:采用SD大鼠,随机分为对照组、对照+DHA组、糖尿病组和糖尿病+DHA组。以链脲佐菌素诱发大鼠糖尿病,DHA组给予DHA(200mg/kg体重)干预,对照组和糖尿病组给予玉米油(0.2 ml/kg体重)。检测各组大鼠机械痛和热痛阈值,ELISA法、PCR法检测背根节中CXCL1含量,免疫荧光法检测CXCR2受体表达情况,Western Blot法检测背根节中JNK磷酸化水平。结果:糖尿病大鼠机械痛敏和热痛敏阈值下降,DHA可在一定程度上缓解大鼠痛反应。糖尿病大鼠背根节JNK磷酸化水平升高,CXCL1水平明显升高,CXCR2受体阳性神经元数量明显升高。DHA干预明显抑制JNK磷酸化水平和CXCR2的表达,降低CXCL1的含量。结论:DHA具有减轻糖尿病神经病理性痛大鼠机械痛敏和热痛敏的作用,其机制可能与抑制背根节JNK磷酸化,降低炎性因子水平有关。
Objective: To investigate the effect of DHA on mechanical hyperalgesia and heat hyperalgesia in diabetic rats with neuropathic pain and to investigate the effect of DHA on JNK phosphorylation and CXCL1 level and its receptor expression in diabetic rat dorsal root ganglion. Methods: SD rats were randomly divided into control group, control + DHA group, diabetes group and diabetes + DHA group. Diabetes was induced by streptozotocin in rats. DHA (200mg / kg body weight) was given to DHA group and corn oil (0.2ml / kg body weight) was given to control group and diabetic group. The mechanical and thermal pain thresholds of rats in each group were detected. The content of CXCL1 in DRG was detected by ELISA and PCR. The expression of CXCR2 receptor was detected by immunofluorescence. The phosphorylation of JNK was detected by Western Blot. Results: The thresholds of mechanical hyperalgesia and thermal hyperalgesia in diabetic rats were decreased. DHA relieved the pain response in rats to some extent. The level of JNK phosphorylation, the level of CXCL1 and the number of CXCR2 receptor positive neurons in diabetic dorsal root ganglia increased significantly. DHA intervention significantly inhibited JNK phosphorylation and CXCR2 expression and decreased CXCL1 content. Conclusion: DHA can reduce the mechanical hyperalgesia and hyperalgesia in diabetic rats with neuropathic pain. The mechanism may be related to the inhibition of JNK phosphorylation and the reduction of inflammatory cytokines in DRG.