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目的观察中药防哮饮对支气管哮喘(哮喘)小鼠血清IL-5、IL-12水平及骨髓嗜酸性粒细胞百分计数(EOS%)的影响。方法将50只BALB/c健康小鼠随机分为5组:正常对照组、哮喘模型组、防哮饮小剂量组、防哮饮大剂量组、布地奈德(BUD)组。用鸡卵白蛋白(OVA)致敏激发造模。中药防哮饮组从小鼠第1次致敏当天起,将防哮饮按小剂量(含生药500 g.L-1)、大剂量(含生药2 500 g.L-1)以灌胃方式给药,每天1次,每次0.4 mL,共26 d。在最后1次激发后48 h处死小鼠,采用双抗体夹心酶联免疫吸附试验(ELISA)检测小鼠血清IL-5、IL-12水平,并镜检其骨髓EOS%。结果哮喘模型组小鼠骨髓EOS%[(8.55±1.55)%]较正常对照组[(3.90±0.94)%]明显升高(P<0.01);IL-5水平[(25.69±4.75)ng.L-1]较正常对照组[(17.50±4.71)ng.L-1]明显升高(P<0.05);IL-12水平[(65.72±8.90)ng.L-1]较正常对照组[(81.60±9.40)ng.L-1]明显降低(P<0.01)。防哮饮大剂量组和BUD组均能显著提高小鼠血清IL-12水平、降低血清IL-5水平及骨髓EOS%(Pa<0.01),但2组比较差异均无统计学意义(Pa>0.05)。与防哮饮大剂量组比较,防哮饮小剂量组效果不显著。结论中药防哮饮通过下调血清IL-5水平、上调血清IL-12水平,从而调节Th1/Th2平衡,达到防治哮喘的目的。
Objective To observe the effect of anti-asthma drink on IL-5 and IL-12 levels in serum and eosinophil percentage in mice with bronchial asthma (asthma). Methods Fifty BALB / c healthy mice were randomly divided into five groups: normal control group, asthma model group, low dose anti - asthma drink group, high dose anti - asthma drink group and budesonide (BUD) group. Sensitized with chicken ovalbumin (OVA). Chinese medicine anti-asthma drink group mice from the first day of sensitization, the anti-asthma drink by small doses (including crude drug 500 gL-1), large doses (with crude drug 2 500 gL-1) by gavage daily administration 1 times, each 0.4 mL, a total of 26 d. Mice were sacrificed at 48 h after the last challenge. Serum levels of IL-5 and IL-12 were detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA), and their bone marrow EOS% was examined. Results The bone marrow EOS% (8.55 ± 1.55)% in asthmatic model group was significantly higher than that in normal control group (3.90 ± 0.94%) (P <0.01). IL-5 level was (25.69 ± 4.75) ng / L-1] was significantly higher than that of the normal control group [(17.50 ± 4.71) ng.L-1] (P <0.05); IL-12 level was (65.72 ± 8.90) ng.L- (81.60 ± 9.40) ng.L-1] (P <0.01). Both anti-asthma drink high-dose group and BUD group could significantly increase serum IL-12 level, decrease serum IL-5 level and bone marrow EOS% (Pa <0.01), but there was no significant difference between the two groups (Pa> 0.05). Compared with the high-dose anti-asthma drink group, anti-asthma drink low-dose group effect is not significant. Conclusion Chinese medicine anti-asthma drink can prevent and treat asthma by down-regulating serum IL-5 level and up-regulating serum IL-12 level, thereby regulating the balance of Th1 / Th2.