[Objective] To establish the high performance liquid chromatogram (HPLC) fingerprint chromatogram of RADIX ACONITI LATERALIS PRAEPARATA and apply it to identify the genuine regional and non-genuine regional medicinal herb.[Method] The HPLC analysis was performed on a Waters XB-C18 (250 mm×4.6 mm,5 μm) with the detection wavelength at 240 nm and column temperature at 30 ℃.Following the injection of 20 μl of sample solution,an elution with the mixture of 40 mmol/L ammonium acetate (adjusted to pH 10.0) and acetonitrile as mobile phase was initiated at a flow rate of 1.0 ml/min,about lasted 70 min.[Result] A total of 22 common peaks were identified from the fingerprints of 6 bathes of genuine regional medicinal herbs,and their similarity was all above 0.926%.From the perspective of peak area ratio of common peaks,there were certain differences in the chemical constituents among different genuine regional medicinal herbs.The 6 batches of non-genuine regional medicinal herbs had 15 common peaks with the similarity all less than 0.879,which were significantly different in chemical constituents.In addition,the chemical constituents were more complicated in genuine regional than non-genuine regional medicinal herb.[Conclusion] The established HPLC fingerprint could distinguish the genuine regional and non-genuine regional medicinal herb,which could provide scientific basis to better control the inner quality of RADIX ACONITI LATERALIS PRAEPARATA. [Objective] To establish the high performance liquid chromatogram (HPLC) fingerprint chromatogram of RADIX ACONITI LATERALIS PRAEPARATA and apply it to identify the genuine regional and non-genuine regional medicinal herb. [Method] The HPLC analysis was performed on a Waters XB-C18 (250 mm × 4.6 mm, 5 μm) with the detection wavelength at 240 nm and column temperature at 30 ° C. After the injection of 20 μl of sample solution, an elution with the mixture of 40 mmol / L ammonium acetate (adjusted to pH 10.0) and acetonitrile as mobile phase was initiated at a flow rate of 1.0 ml / min, about lasted 70 min. [Result] A total of 22 common peaks identified from the fingerprints of 6 bathes of genuine regional medicinal herbs, and their similarity was all above 0.926% .From the perspective of peak area ratio of common peaks, there were certain differences in the chemical constituents among different genuine regional medicinal herbs. 6th Batch of non-genuine regional medicinal herbs had 15 com mon peaks with the similarity all less than 0.879, which were significantly different in chemical constituents. In addition, the chemical constituents were more complicated in genuine regional than non-genuine regional medicinal herb. [Conclusion] The established HPLC fingerprint could distinguish the genuine regional and non-genuine regional medicinal herb, which could provide scientific basis to better control the inner quality of RADIX ACONITI LATERALIS PRAEPARATA.