论文部分内容阅读
神经元之间的缝隙连接蛋白Cx32是形成电突触的结构基础，电突触参与惊厥时神经元的同步化放电过程。为探讨惊厥对脑内神经元Cx32基因表达的影响，以遗传性癫痫易感大鼠P77PMC为模型，采用原位杂交的方法观察P77PMC大鼠听源性惊厥后脑内Cx32基因表达的变化。原位杂交结果显示：惊厥后在大脑皮层，海马脑区Cx32mRNA表达呈时间依赖性上调，惊厥后4h表达量明显增加，24h达高峰，结果提示：惊厥能上调神经元Cx32mRNA表达，从而可能使神经元之间的电突触联系增加，有利于神经元的同步化放电，加重惊厥的发生发展。
The connexin Cx32 between neurons is the structural basis for the formation of electrical synapses involved in the synchronized firing of neurons during convulsions. To investigate the effect of convulsion on the expression of Cx32 in neurons of brain in rats, the expression of Cx32 gene in auditory seizure brain of P77PMC rats was observed by in situ hybridization in P77PMC with genetic predisposition to epilepsy. The result of in situ hybridization showed that the expression of Cx32mRNA in cerebral cortex and hippocampus was increased in a time-dependent manner after seizure. The expression of Cx32mRNA increased significantly at 4h after seizure and peaked at 24h, indicating that convulsion could up-regulate the expression of Cx32mRNA in neurons, The synaptic contact between the elements increases, which is conducive to the synchronization of neurons to discharge, aggravating the occurrence and development of convulsions.