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目的 应用神经特殊染色技术及电生理学方法 ,探讨周围神经卡压松解后神经纤维的再生修复和神经传导功能的变化。方法 在大鼠坐骨神经卡压模型基础上 ,将 6 0只 SD成年雄性大鼠随机等分为四组。A组 :仅去除卡压 ;B组 :去除卡压后切开神经外膜 ;C组 :去除卡压后神经外膜下周围注射利美达松 (0 .5 m g/ kg) ;D组 :去除卡压后切开神经外膜 ,然后在神经周围注射利美达松 (0 .5 mg/ kg)。于去除卡压后 1、2、3、4和 5周行运动神经传导速度 (MNCV)及潜伏期 (L an)检测 ,定期取材进行组织学观察。结果 神经卡压松解后 2周 ,各组 MNCV及 L an均有明显恢复 ,至第 5周 ,C、D组电生理变化明显优于 A、B组 ,其中 C组恢复最明显 (P<0 .0 5 ) ,而 A与 B组相比 ,无统计学差异 (P>0 .0 5 )。组织学观察到卡压松解后 3周 ,C、D组均可见大量再生神经纤维。结论 卡压去除后可明显改善神经传导功能 ,而松解后神经周围给予利美达松有助于损伤神经的结构再生和功能恢复。
OBJECTIVE: To study the changes of nerve fiber regeneration and repair and neurotransmission function after nerve entrapment and release by peripheral nerve using special nerve staining and electrophysiological methods. Methods Based on the rat model of sciatic nerve compression, 60 male SD rats were randomly divided into four groups. In group A, the cardiotomy was only removed; in group B, the epineurium was excised after compression; in group C, lemethasone (0.5 mg / kg) After removal of the compression, the epineurium is incised and then injected with lemethasone (0.5 mg / kg) around the nerve. Motor nerve conduction velocity (MNCV) and latency (L an) were measured at 1, 2, 3, 4 and 5 weeks after removal of cardiopulmonary resuscitation, and histological observation was performed regularly. Results The changes of MNCV and L an in each group were obviously recovered 2 weeks after the nerve card was released. At the 5th week, the electrophysiological changes in group C and D were better than those in group A and B, and the recovery was the most obvious in group C (P < 0 .0 5), while A and B group, no significant difference (P> 0.05). Histology observed after 3 weeks of card-pressure release, C, D group can be seen a large number of regenerated nerve fibers. Conclusions Cardiac pressure removal can obviously improve the nerve conduction function, and the release of lemethasone around the nerve after release can help the nerve regeneration and functional recovery.