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目的探讨癌细胞ｍｔＤＮＡ基因编码区的结构特征。方法采用ＰＣＲ和ＰＣＲ产物ＨａｅⅢ限制性片段长度多态性分析方法，分析了６株人癌细胞系、８例癌患者及８例正常人白细胞线粒体ＣｏⅡ和ＡＴＰａｓｅ６基因的缺失和限制酶谱变化。结果在６个癌细胞系、８例癌患者和８例健康成人白细胞ｍｔＤＮＡ中，两个基因片段均未出现缺失现象；６个癌细胞系及８例癌患者白细胞ｍｔＤＮＡＣｏⅡ和ＡＴＰａｓｅ６基因ＰＣＲ扩增片段ＨａｅⅢ的酶切位点一致，且与人ｍｔＤＮＡ剑桥序列相应区段ＨａｅⅢ酶切位点完全同源，与８例健康成人白细胞ｍｔＤＮＡ相应基因片段ＨａｅⅢ酶切位点比较，也具有非常高的同源性。结论癌细胞ｍｔＤＮＡ基因编码区结构非常稳定。
Objective To investigate the structural features of the coding region of mtDNA gene in cancer cells. Methods The deletions and restriction enzyme patterns of the mitochondrial CoII and ATPase6 genes in white blood cell of 6 human cancer cell lines, 8 cancer patients and 8 normal humans were analyzed by PCR and PCR products Hae III restriction fragment length polymorphism analysis. Results In the mtDNA of white blood cell of 6 cancer cell lines, 8 cases of cancer patients and 8 healthy adults, there was no deletion of the two gene fragments; the PCR amplification fragments of mtDNACoII and ATPase6 gene of white blood cells in 6 cancer cell lines and 8 cancer patients HaeIII has the same restriction enzyme sites and is homologous to HaeIII restriction site in the corresponding segment of the human mtDNA Cambridge sequence. It is also highly homologous to HaeIII restriction site of 8 healthy adult leukocyte mtDNA counterparts. Sex. Conclusion The coding region structure of mtDNA gene in cancer cells is very stable.