论文部分内容阅读
酶联免疫分析法(Enzyme-linked Immunosorbent Assay,ELISA)是将抗原与抗体的特异性免疫反应和酶的高效催化作用有机结合起来的检测技术。具体是将抗原或抗体结合到某固相载体(保持其免疫活性),使受检标本和酶标抗体或抗原与固相载体表面的抗原或抗体起反应,然后洗涤,使固相载体上形成的抗原抗体复合物与其他物质分开。因最后结合在固相载体上的酶量与标本中受检物质的量成一定的比例,所以可根据加入底物后,酶催化反应产生有色物质颜色的深浅进行定性或定量分析。如今,尽管各种新型食品安全检测技术不断涌现,ELISA技术仍因其操作程序的规范化、简单化和检测的高灵敏性,表现出独特的优势,并在药物残留、生物毒素、食品添加剂和人畜共患疾病病原体的快速检测与分析等食品安全检测领域中得到广泛应用。
Enzyme-linked Immunosorbent Assay (ELISA) is a combination of antigen-antibody-specific immunoreactions and enzyme-catalyzed high-performance catalysis. Specifically, the antigen or antibody is bound to a solid support (keeping its immunocompetence) so that the test sample and the enzyme-labeled antibody or antigen react with the antigen or antibody on the surface of the solid support and then washed to form a solid support The antigen-antibody complex is separated from other substances. Due to the amount of the enzyme finally bound to the solid support and the amount of the substance to be tested in the sample, a qualitative or quantitative analysis can be performed according to the depth of color of the colored material generated by the enzyme-catalyzed reaction after addition of the substrate. Today, despite the emerging availability of new food safety assays, ELISA technology continues to offer unique advantages due to the standardization, simplification and high sensitivity of its protocols, as well as drug residues, biotoxins, food additives, Comorbid disease pathogens such as rapid detection and analysis of food safety testing has been widely used in the field.