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利用生物信息网络数据库,对甘蓝型油菜紫色酸性磷酸酶基因(PAP)类似物编码的蛋白进行生物信息学分析,以预测该蛋白的理化性质、分子结构与生物学功能。结果表明:该基因编码的蛋白含481个氨基酸,其蛋白的分子式为C2520H3714N666O719S19,分子量为55.45kDa,等电点为6.35,为亲水性分泌型蛋白,并且不稳定;存在3个跨膜螺旋区域及3个卷曲螺旋结构,含有1个信号肽、39个潜在磷酸化位点和3个N-糖基化修饰,存在6种类型特定的功能位点,主要定位于高尔基体;氨基酸序列同源性分析表明,该基因编码的蛋白与甘蓝型油菜PAP12家族具有较近的亲缘关系,直系同源于甘蓝型油菜PAP12-5;二级结构主要为无规则卷曲、延伸链和α?螺旋,具有较为明显的10个螺旋和26个折叠;具有PAP的保守结构域,属于金属磷酸二酯酶超家族成员;根据保守结构域及功能位点分析发现该基因属于紫色酸性磷酸酶类,可能属于甘蓝型油菜PAP基因家族成员,预测其编码产物在植物磷高效利用与吸收中发挥重要的生理功能。生物信息学分析及结合蛋白的结构与功能预测分析可为深入研究甘蓝型油菜PAP提供理论指导。
The bioinformatics analysis of the protein encoded by the purple acid phosphatase (PAP) analogue of rapeseed (Brassica napus) was carried out by bioinformatics network database to predict the physicochemical properties, molecular structure and biological function of the protein. The results showed that the protein encoded by this gene contains 481 amino acids. Its protein has the molecular formula of C2520H3714N666O719S19, the molecular weight of 55.45kDa and the isoelectric point of 6.35. It is a hydrophilic secreted protein and is unstable. There are three transmembrane helical regions And three coiled-coil structures, including one signal peptide, 39 potential phosphorylation sites and 3 N-glycosylation modifications. There are 6 types of specific functional sites, which are mainly located in the Golgi apparatus. Amino acid sequence homology Sex analysis showed that the protein encoded by this gene has a closer genetic relationship with the PAP12 family of Brassica napus and orthologous to PAP12-5 of Brassica napus. The secondary structure is mainly random curl, extension chain and α-helix with More obvious 10 helix and 26 folds; a conserved domain with PAP belongs to the metal phosphodiesterase superfamily member; according to the analysis of conserved domains and functional sites, it is found that the gene belongs to the purple acid phosphatase class, which may belong to cabbage Type rape PAP gene family members predicted that the encoded products play an important physiological function in the efficient use and absorption of plant phosphorus. Bioinformatics analysis and structural and functional predictive analysis of the binding protein provide theoretical guidance for further study on PAP in Brassica napus.