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目的：检测粘放菌５５１９ Ｉ型菌毛的纯度及 Ｗｅｓｔｅｒｎ Ｂｌｏｔｔｉｎｇ 方法的特异性。方法：采用 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 电泳和 Ｗｅｓｔｅｒｎ Ｂｌｏｔｔｉｎｇ 免疫印迹法。结果： 粘放菌 ５５１９ Ｉ型菌 Ｍｒ 大部分为５４ ０００，少部分为３８ ０００。 Ｗｅｓｔｅｒｎ Ｂｌｏｔｔｉｎｇ 检测结果与 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ结果相一致。结论： Ｗｅｓｔｅｒｎ Ｂｌｏｔｔｉｎｇ 在粘放菌菌毛鉴定中具有高度特异性。
Objective: To test the purity of 5519 type I pili and the specificity of Western Blotting. Methods: S D S P A G E electrophoresis and Western Blotting immunoblotting. Results: The majority of Myxobacteriian 5519 Type I bacteria was 54 000, with a small proportion of 38 000. Western Blotting test results and S D S-P A G E results are consistent. Conclusion: Western Blotting is highly specific in the identification of mucilaginous pili.