论文部分内容阅读
运用序批式生物絮凝反应器,研究不同混合液悬浮固体浓度(MLSS,1 500 mg/L、3 000 mg/L和5 000mg/L)下反应器对循环水养殖系统吉富罗非鱼(GIFT Oreochromis niloticus)养殖废水的处理效果。结果表明:反应器内氨氮(TAN)、亚硝氮(NO-2-N)和硝氮(NO-3-N)出水浓度分别为(0.29~0.39)mg/L、(0.005~0.006)mg/L、(7.11~7.60)mg/L,平均去除率分别为82.20%~86.20%、98.40%±0.89%、38.40%~40.00%(P>0.05),体积去除负荷为(2.51~2.64)g/(m3·d)、0.56 g/(m3·d)、(8.52~9.78)g/(m3·d);溶解性无机氮(DIN)的去除率为43.20%~44.60%,体积去除负荷为(10.25~11.61)g/(m3·d)。三组絮体蛋白质含量差异不显著,分别为30.00%±1.32%、29.87%±0.67%、31.00%±0.75%;粗脂肪含量分别为9.51%±0.94%、4.37%±0.42%、3.65%±0.22%,MLSS 1500 mg/L组显著高于其他两组(P<0.05)。微生物群落结构分析表明反应器中生物絮体主要为变形菌门(44.66%、44.51%、44.29%),其次是拟杆菌门(13.89%、13.98%、14.07%);优势菌属包括Alishewanella、Blastocatella、Amaricoccus、Rhodobacteraceae_unclassified、Terrimonas、Devosia等。实验表明中试生物絮凝反应器具有较好的脱氮效果,有助于实现养殖废水的资源化应用。
The sequencing batch bioflocculant reactor was used to study the effects of reactors on the recycle system of Grifola tilapia (GIFT) under different mixed liquor concentrations (MLSS, 1 500 mg / L, 3 000 mg / L and 5 000 mg / L) Oreochromis niloticus aquaculture wastewater treatment effect. The results showed that the effluent concentrations of TAN, NO-2-N and NO-3-N in the reactor were (0.29-0.39) mg / L, (0.005-0.006) mg /L,(7.11~7.60)mg/L, the average removal rates were 82.20% -86.20%, 98.40% ± 0.89%, 38.40% -40.00%, respectively (P> 0.05) / (m3 · d), 0.56 g / (m3 · d) and (8.52 ~ 9.78) g / (m3 · d), respectively. The removal rates of dissolved inorganic nitrogen (DIN) were 43.20% ~ 44.60% (10.25 ~ 11.61) g / (m3 · d). The protein content of the three groups was not significantly different (30.00% ± 1.32%, 29.87% ± 0.67%, 31.00% ± 0.75%, respectively); the crude fat contents were 9.51% ± 0.94%, 4.37% ± 0.42%, 3.65% ± 0.22%, MLSS 1500 mg / L group was significantly higher than the other two groups (P <0.05). Microbial community structure analysis showed that the bioflocs in the reactor were mainly Proteobacteria (44.66%, 44.51%, 44.29%), followed by Bacteroidetes (13.89%, 13.98%, 14.07%). The dominant bacteria included Alishewanella, Blastocatella , Amaricoccus, Rhodobacteraceae_unclassified, Terrimonas, Devosia and others. Experiments show that the pilot biological flocculation reactor has better denitrification effect, which is helpful for the resource utilization of aquaculture wastewater.