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目的比较人脐血源基质细胞(hUCBDSCs)与骨髓基质细胞(hBMSCs)对造血功能损伤的修复作用。方法取63只BALB/c小鼠均给予6.0 Gy60Co射线全身照射,随机分为hUCBDSCs组(n=21)、hBMSCs组(n=21)和对照组(生理盐水组)(n=21),分别于移植后1、3、5、7、10、14、21 d检测1次外周血细胞,于照射后7和21 d分别制备股骨骨髓涂片观察骨髓细胞形态学改变,收集小鼠骨髓细胞并作集落培养。结果 3组小鼠移植后白细胞、血小板、红细胞在3～5 d降至最低,hUCBDSCs组白细胞和血小板均从7 d开始回升,10 d后回升速度与程度提高,21 d时回升接近未照射水平,hBMSCs组从7 d开始回升,回升趋势与hUCBDSCs组相似,21 d后回升程度低于未照射水平,对照组从7 d后开始回升,回升较慢,21 d回升程度低于实验组(P<0.05);hUCBDSCs组,hBMSCs组及对照组红细胞均在3 d时降至最低,21 d后恢复,但实验组较对照组回升更快,(P<0.05),hUCBDSCs组与hBM-SCs无甚差异(P>0.05)。集落培养CFU-E、BFU-E、CFU-GM、CFU-GMEM,hUCBDSCs组21 d为(113±6)、(167±6)、(131±6)、(14±4)个,hBMSCs组(46±6)、(141±11)、(118±18)、(10±1)个,对照组为(87±3)、(122±4)、(84±7)、(5±1)个(P<0.05)。hUCBDSCs组所有小鼠均存活。结论 hUCBDSCs较hBMSCs修复造血功能损伤和血小板恢复的作用更佳,hUCBDSCs移植是1种安全有效的促进造血损伤修复的方法。
Objective To compare the repair effects of human umbilical cord blood stromal cells (hUCBDSCs) and bone marrow stromal cells (hBMSCs) on hematopoietic injury. Methods Totally 63 BALB / c mice were given whole body irradiation of 6.0 Gy 60Co and were randomly divided into hUCBDSCs group (n = 21), hBMSCs group (n = 21) and control group (n = 21) Peripheral blood cells were detected at 1, 3, 5, 7, 10, 14 and 21 days after transplantation. The bone marrow smears were prepared at 7 and 21 days after irradiation to observe the morphological changes of bone marrow cells and collect mouse bone marrow cells for Colony culture. Results After leukocyte, platelet and erythrocyte transplantation, the number of leucocytes, platelets and erythrocytes in the three groups of mice decreased to the lowest after 3 to 5 days. The white blood cells and platelets in hUCBDSCs group recovered from day 7 after the transplantation. The recovery rate and degree increased after 10 days. , the hBMSCs group started to recover from 7th day, and the rising trend was similar to that of hUCBDSCs group. After 21 days, the recovery degree of hBMSCs group was lower than that of non-irradiated group. The control group started to recover after 7 days, <0.05). In the hUCBDSCs group, hBMSCs group and control group, erythrocytes were all reduced to the lowest level on the 3rd day and resumed after 21 days, but the recovery of the experimental group was faster than that of the control group (P <0.05) Very different (P> 0.05). The numbers of hBMSCs in group of hUCBDSCs were (113 ± 6), (167 ± 6), (131 ± 6) and (14 ± 4) days after colonies were cultured in CFU-E, BFU-E, CFU-GM, CFU- (46 ± 6), (141 ± 11), (118 ± 18) and (10 ± 1) in the control group were (87 ± 3), (122 ± 4), (84 ± 7) and ) (P <0.05). All mice in the hUCBDSCs group survived. Conclusion hUCBDSCs are more effective than hBMSCs in repairing hematopoietic injury and platelet recovery. HUCBDSCs transplantation is a safe and effective method to promote hematopoietic repair.