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目的快速准确地检测副溶血性弧菌(VP)和伤寒沙门氏菌(ST)。方法:根据VP的tdh基因和ST的HI-d基因顺序设计二对引物,通过聚合酶链反应检测食品或其它待检样品中的VP和ST,将扩增产物电泳。结果:分别出现与已知阳性对照细菌条带大小一致的202和458bp的tdh和HI-d基因片断的条带,判定该样品中相应细菌为阳性。结论:本法特异性强、灵敏度高,能在24小时内出结果,可适用于食品或其它样品中VP和ST的快速检验。
Objective To rapidly and accurately detect Vibrio parahaemolyticus (VP) and Salmonella typhi (ST). Methods: Two pairs of primers were designed according to the sequence of tdh gene of VP and ST-HI, and PCR products of VP and ST in food or other samples were detected by polymerase chain reaction (PCR). Results: The bands of 202 and 458 bp tdh and HI-d gene fragments with the same size as that of the known positive control bacteria appeared respectively, and the corresponding bacteria in this sample were found to be positive. Conclusion: This method is of high specificity and high sensitivity, and can output results in 24 hours. It is suitable for the rapid test of VP and ST in food or other samples.