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目的研究煤工尘肺患者血清高迁移率族蛋白B-1(HMGB1)与肺功能的关系,为煤矽肺的早期防治提供理论依据。方法选择某矿务局体检机构的不同期别煤工尘肺患者作为人群试验采集对象,共1 126例,其中0+期624例,Ⅰ期324例,Ⅱ期120例,Ⅲ期58例。采集患者血清,采用酶联免疫吸附的方法测量HMGB1的表达,采用肺功能仪HI-801进行肺功能测定。均数的两两比较采用bonferroni法,对应的检验水准调整为α=0.008;相关分析采用Spearman秩相关,检验水准α=0.05。结果 (1)Ⅱ期煤工尘肺患者血清HMGB1浓度与其他3期相比较均有统计学差异(P均<0.008)。(2)随着尘肺期别增加,患者血清中HMGB1浓度逐渐升高(P<0.008)。(3)煤工尘肺患者血清HMGB1浓度与反映肺功能的3个指标VC、FVC、FEV1均呈负相关,相关系数分别为-0.734、-0.912、-0.893(P均<0.05)。提示随血清HMGB1浓度的升高,VC、FVC、FEV1均降低。结论煤工尘肺患者血清HMGB1浓度的升高可反映肺功能的损伤。
Objective To study the relationship between serum high mobility group box-1 (HMGB1) and pulmonary function in patients with coal worker’s pneumoconiosis, and to provide a theoretical basis for early prevention and treatment of coal silicosis. Methods A total of 1 126 cases of coal miners pneumoconiosis were selected as the subjects of the population test in different phases of the medical institution of a mining bureau. There were 624 cases in stage 0+, 324 cases in stage I, 120 cases in stage II and 58 cases in stage III. The serum of patients was collected, the expression of HMGB1 was measured by enzyme-linked immunosorbent assay, and the pulmonary function test was performed with pulmonary function instrument HI-801. The pairwise comparison of mean was performed by the bonferroni method, the corresponding test level was adjusted to be α = 0.008; Spearman’s rank correlation was used for the correlation analysis, and the test level α = 0.05. Results (1) The level of serum HMGB1 in patients with stage Ⅱ pneumoconiosis was significantly different from that in other three stages (all P <0.008). (2) With the increase of pneumoconiosis stage, serum HMGB1 concentration gradually increased (P <0.008). (3) The concentration of serum HMGB1 in patients with coal worker’s pneumoconiosis was negatively correlated with VC, FVC and FEV1 which reflect the lung function. The correlation coefficients were -0.734, -0.912 and -0.893 respectively (all P <0.05). The results suggest that with the increase of serum HMGB1 concentration, VC, FVC and FEV1 are decreased. Conclusion The increase of HMGB1 concentration in coal workers’ pneumoconiosis can reflect the impairment of lung function.