无论从细胞生物学角度或是从临床肿瘤学角度,对乏氧肿瘤细胞的研究都很重要。我们的观察表明,乏氧48～72小时的Hela细胞群体中仍有少数细胞维持增殖的能力。乏氧HeLa细胞的形态学改变主要有胞浆内出现空泡,并在细胞核与细胞浆间有巨大间隙的形成,扫描电镜下乏氧早期HeLa细胞有形似“不正常”的伪足形成,微绒毛亦较粗短,其意义尚有待深入探讨。 From the perspective of cell biology or clinical oncology, research on hypoxic tumor cells is important. Our observations indicate that there is still a small number of cells in the Hela cell population that are 48 hours to 72 hours of hypoxia to maintain their ability to proliferate. The morphological changes of hypoxic HeLa cells are mainly vacuoles in the cytoplasm and large gaps between the nucleus and cytoplasm. The early hypoxic HeLa cells are shaped by the scanning electron microscopy to form pseudopodia that are “unusual”. The villi are also relatively short and their significance has yet to be further explored.