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目的:观察富含n-3多不饱和脂肪酸(n-3 PUFA)的肠外营养(PN)能否通过鞘氨醇激酶1(Sph K1)和磷酸鞘氨醇受体(S1P)途径影响机体的炎性反应。方法:对大鼠实施颈外静脉置管,2 d后行盲肠结扎穿孔,然后随机分为等渗盐水组(静脉输注等渗盐水,n=20)、鱼油组(鱼油+PN,n=20)和大豆油组(PN,n=20),随后行PN支持5 d。另设正常对照组(n=10)实施假手术。检测血清IL-10和高迁移率族蛋白(HMGB-1)浓度、脾组织中Sph K1、S1PR1、S1PR3表达情况以及Sph K1酶活性。结果:与对照组比,等渗盐水组大鼠Sph K1 mRNA、蛋白表达和酶活性明显升高,S1PR3 mRNA和蛋白表达也上调;鱼油组血清IL-10和HMGB-1浓度显著降低;Sph K1 mRNA、蛋白表达和酶活性显著受抑制;S1PR3 mRNA和蛋白表达也受抑。结论:n-3 PUFA的PN可通过抑制Sph K1和S1PR3通路改善脓毒症大鼠的炎性反应。
PURPOSE: To investigate whether parenteral nutrition (PN) enriched in n-3 polyunsaturated fatty acids (n-3 PUFA) affects the body via Sph K1 and sphingosinephosphate receptors (S1P) Inflammatory reaction. Methods: The external jugular vein catheterization was performed in rats and cecum ligation and perforation were performed 2 days later. The rats were randomly divided into isotonic saline group (n = 20 for intravenous infusion of isotonic saline, fish oil + PN, n = 20) and soybean oil (PN, n = 20) followed by PN support for 5 days. Another normal control group (n = 10) to implement sham surgery. Serum levels of IL-10 and HMGB-1, Sph K1, S1PR1, S1PR3 and Sph K1 enzyme activity in spleen tissue were measured. Results: Compared with the control group, the mRNA and protein expressions of Sph K1 and the activity of Sph K1 protein were significantly increased and the expressions of S1PR3 mRNA and protein were up-regulated in the rats in isotonic saline group. The levels of IL-10 and HMGB-1 in fish oil group were significantly decreased. mRNA, protein expression and enzyme activity were significantly inhibited; S1PR3 mRNA and protein expression was also inhibited. CONCLUSION: PN with n-3 PUFA can ameliorate the inflammatory response in septic rats by inhibiting the Sph K1 and S1PR3 pathways.