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作者以前曾设想利用粘果山羊草（Ａｅ．ｋｏｔｓｃｈｙｉ）的Ｓｖ型细胞质与１ＢＬ－１ＲＳ染色体互作产生的雄性不育培育杂交小麦（Ｔｏｒｉｙａｍａ等，１９９１，１９９３），在此设想中，雄性不育保持系可通过两个渠道获得，即回交法（Ｎｏｎａｋａ等，１９９３）和花药培养法．本文报道花药培养的结果。用 １ＢＬ－１ＲＳ染色体携带者９１１－Ｂ－８－１０（简称ｓｔ．９１１）与１３个日本品种杂交，用 Ｆ１及 Ｂ１Ｆ１植株的花药进行培养，Ｆ１的胚形成频率高于亲本（日本品种），Ｂ１Ｆ１的胚形成频率低于 Ｆ１代。ｓｔ．９１１和Ｆ１代较高的胚形成频率部分地得益于 １ＢＬ－１ＲＳ染色体．因为抗叶锈病基因Ｌｒ位于１ＲＳ染色体臂上，所以在再生单倍体植株上人工接种叶锈病菌（２１Ｂ小种）可以选出抗病个体。用抗叶锈的加倍单倍体与带有１Ｂ染色体的（Ｓｖ）－中国春和带有１ＢＬ－１ＲＳ染色体的（Ｓｖ）－Ｓａｉｍｏｎ杂交，前者的Ｆ１表现出较高的自交可育性，而后者的Ｆ１表现出完全雄性不育，这说明，通过花药培养获得的抗叶锈病的加倍单倍体系可以用作雄性不育系的保持系。
The authors had previously envisaged breeding male sterile wheat (Toriyama et al., 1991, 1993) using male sterility produced by the interaction of the Sv-type cytoplasm of Ae.kotschyi with the 1BL-1RS chromosome, in which it is assumed that male sterility Retention can be obtained through two channels, Backhaul (Nonaka et al., 1993) and Anther culture. This article reports the results of anther culture. The F1BL and F1F1 plants were cultured with11BL-1RS chromosome carriers 911-B-8-10 (referred to as st.911 for short) and 13 Japanese cultivars. The frequency of F1 embryogenesis was higher than that of the parent (Japanese cultivar) The embryogenesis frequency of B1F1 was lower than F1 generation. st. The higher frequency of embryogenesis in the 911 and F1 generations partially benefits from the 1BL-1RS chromosome. Because the leaf rust resistance gene Lr is located on the 1RS chromosomal arm, resistant individuals can be selected by artificially inoculating leaf rust (21B race) on regenerated haploid plants. F1 hybrids with the 1BL-1RS chromosome with (Sv) -Chinese Spring with 1B chromosome and (Sv) -Saimon with 1BL-1RS were crossed with doubled haploid of leaf rust, the former F1 showed higher inbred fertility, While F1 of the latter showed complete male sterility, indicating that doubled haploid system of leaf rust resistance obtained by anther culture can be used as a maintainer of male sterile lines.