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用一阶导数光谱法测定无环鸟苷霜中无环鸟苷的含量，可消除赋形剂的干扰。测定波长为240．6um和286．0nm，平均回收率为100．1％，CV＝0．58％。
Determination of acyclovir in the acyclovir cream by first derivative spectroscopy can eliminate the interference of the excipients. The measurement wavelength was 240.6um and 286.0nm, the average recovery was 100.1%, CV = 0.58%.