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为研究老年时肿瘤坏死因子（ＴＮＦ－α）分泌及表达的改变，在内毒素（ＬＰＳ）１．０μｇ／ｍｌ刺激大鼠单核／巨噬细胞后，用酶联免疫法（ＥＬＩＳＡ）测定培养液中ＴＮＦ－α含量，用半定量逆转录－聚合酶链反应（ＲＴ－ＰＣＫ）测定ＴＮＦ－αｍＲＮＡ。同时测定培养液中一氧化氮（ＮＯ）和前列腺素Ｉ２（ＰＧＩ２）的含量。结果显示：老年鼠ＴＮＦ－α分泌量及其ｍＲ－ＮＡ明显高于青年鼠。ＮＯ产量在老年鼠与青年鼠之间无明显差异。老年鼠ＰＧＩ２分泌明显低于青年鼠。由于ＰＧ能抑制ＴＮＦ－α释放，从而推测，ＰＧＩ２产生能力的降低可能是老年大鼠单核／巨噬细胞ＴＮＦ－α分泌量明显高于青年鼠的原因之一。
To study changes in the secretion and expression of tumor necrosis factor (TNF-α) in the elderly, rat monocyte/macrophages were stimulated with endotoxin (LPS) 1.0 μg/ml, and cultured by enzyme-linked immunosorbent assay (ELISA). The content of TNF-α in the solution was measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCK). At the same time, the contents of nitric oxide (NO) and prostaglandin I2 (PGI2) in the culture fluid were measured. The results showed that the secretion of TNF-α and its mR-NA were significantly higher in younger mice than in younger rats. There was no significant difference in NO production between old and young rats. PGI2 secretion in aged mice was significantly lower than in young rats. Since PG can inhibit TNF-α release, it was hypothesized that the decreased production of PGI2 may be one of the reasons that the secretion of TNF-α in monocytes/macrophages of aged rats is significantly higher than that of young rats.