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目的观察脓毒症大鼠心肌组织的病理改变、血压的变化以及氧化应激的有关指标的变化,分析氧化应激与脓毒症大鼠心肌损伤的关系。方法取30只SD大鼠随机分为两组,对照组10只,实验组20只,实验组再分2个亚组,每组10只,分别于术后6h和12h颈动脉采血并处死摘取心脏,并取部分心脏HE染色,显微镜下观察心肌损伤的情况。分别检测对照组和实验组6h、12h大鼠血清及心肌组织的丙二醛(MDA)、大鼠晚期氧化蛋白产物(AOPP)和大鼠晚期糖基化终产物(AGEs)。结果①脓毒症组大鼠心肌病理学改变包括间质充血水肿、炎性细胞浸润,心肌纤维断裂分层,核膜破裂,细胞基质稀少,心肌细胞空泡变性,嗜酸性变,而对照组大鼠心肌无明显病变。与对照组相比,脓毒症组大鼠血清和心肌组织中多项氧化应激指标在12h后均有明显升高。结论氧化应激参与了脓毒症大鼠心肌损伤的发生。
Objective To observe the changes of pathological changes, blood pressure and oxidative stress in myocardium of septic rats, and to analyze the relationship between oxidative stress and myocardial injury in septic rats. Methods Thirty SD rats were randomly divided into two groups, 10 in the control group and 20 in the experimental group. The experimental group was subdivided into 2 subgroups, 10 in each group. The carotid arteries were harvested at 6h and 12h after operation, respectively. Take the heart, and take part of the heart HE staining, microscopic observation of myocardial injury. The levels of MDA, AOPP and AGEs in serum and myocardium of 6h and 12h rats in control group and experimental group were detected respectively. Results ① The pathological changes of myocardium in sepsis group included interstitial congestion and edema, inflammatory cell infiltration, myocardial fibrosis, ruptured nuclear membrane, sparse cell matrix, degeneration of cardiomyocytes and eosinophilic changes in the control group No obvious myocardial lesions in rats. Compared with the control group, multiple indicators of oxidative stress in serum and myocardial tissue of sepsis group were significantly increased after 12h. Conclusion Oxidative stress is involved in myocardial injury in septic rats.