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目的研究灭活后的柯萨奇病毒Ｂ１（ＣｏｘｓａｋｉｅｖｉｒｕｓＢ１，ＣＶＢ１）在原代培养骨骼肌细胞中，对报道基因ｐＣＤＮＡ３－ＬａｃＺ转移效率的影响。方法用聚乙二醇沉淀和蔗糖低温超速离心法提纯并用β－丙内酯灭活病毒，将灭活ＣＶＢ１、转铁蛋白聚赖氨酸（ＴｆｐＬ）和报道基因设计成不同浓度组合，观察肌肉细胞中β－半乳糖苷酶染色阳性的细胞数。结果灭活ＣＶＢ１＋ＴｆｐＬ＋ＬａｃＺ在肌肉细胞中使基因转移效率由ＴｆｐＬ＋ＬａｃＺ组的不到１％提高到１５％～２０％。结论灭活ＣＶＢ１也能像腺病毒一样促进基因转移，ＣＶＢ１的嗜肌肉特性，为在肌肉组织中研究靶向明确的新载体提供了实验依据。
Objective To investigate the effect of inactivated Coxsakievirus B1 (CVB1) on the efficiency of reporter gene pCDNA3-LacZ in primary cultured skeletal muscle cells. Methods The virus was purified by polyethylene glycol precipitation and sucrose low temperature ultracentrifugation and the virus was inactivated by β-propiolactone. The inactivated CVB1, TfpL and reporter gene were designed into different concentrations to observe the muscle Β-galactosidase staining positive cells in the cell number. Results Inactivation of CVB1 + TfpL + LacZ increased gene transfer efficiency from less than 1% of the TfpL + LacZ group to 15% to 20% in muscle cells. Conclusion Inactivation of CVB1 can also promote gene transfer like adenovirus. The muscle-invading characteristics of CVB1 provide experimental basis for studying new targeting vectors in muscle tissues.