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目的观察重庆地区汉族人群CD14基因启动子区-159单核甘酸多态性(single nuc leotide polymorph ism,SNP)分布特征,及其与CD14表达和脂多糖(lipopolysaccharide,LPS)反应的关系。方法选择298例重庆地区汉族健康个体,采用限制性片段长度多态性法(restrictionfragm ent lengthpoly-morph ism,RFLP)进行基因分型,检测LPS刺激前后外周血单核-巨噬细胞mCD14荧光强度和血浆sCD14浓度。结果重庆地区汉族人群CD14-159 T/T、T/C、C/C基因型频率分别为32.21%、50.67%和17.11%,样本符合Hardy-W e inberg平衡,正常情况下,不同基因型mCD14水平差异不明显,而sCD14浓度有显著性差异(P<0.05),LPS刺激能增强mCD14和sCD14的表达,但各基因型的增强幅度没有明显差异。结论重庆地区汉族人群CD14-159 SNP等位碱基以T为主,T→C变异可抑制sCD14的表达,但对mCD14表达和LPS刺激反应的影响可能不明显。
Objective To investigate the distribution of CD15 gene promoter region -159 single nucleotide polymorphism (SNP) and its relationship with CD14 expression and lipopolysaccharide (LPS) response in Han nationality in Chongqing area. Methods A total of 298 Han healthy individuals from Chongqing were enrolled in this study. The genotypes were determined by restriction fragment length polymorphism (RFLP). The fluorescence intensity of mCD14 in peripheral blood mononuclear macrophages Plasma sCD14 concentration. Results The frequencies of CD14-159 T / T, T / C and C / C genotypes in Han population of Chongqing were 32.21%, 50.67% and 17.11%, respectively. The samples were in Hardy-W einberg equilibrium. Under normal conditions, the genotypes of mCD14 The level of sCD14 was significantly different (P <0.05). LPS stimulated the expression of mCD14 and sCD14, but there was no significant difference in the amplitudes of each genotype. Conclusion The predominant alleles of CD14-159 SNP in Han population of Chongqing population are mainly T, while T → C mutation can inhibit the expression of sCD14, but the effect on mCD14 expression and LPS stimulation may not be obvious.