论文部分内容阅读
为了在实验上分析人正常胃上皮细胞与癌变细胞的显微后向散射光谱的区别,为临床医学检测提供实验依据,在光纤共聚焦显微成像技术基础上,结合细胞散射理论,建立了一套基于光纤共聚焦的细胞检测显微光谱分析装置,能够同时获取细胞显微图像和显微光谱医学信息。利用本装置测量了贴壁人正常和癌变胃上皮细胞,得到细胞水平的显微光谱特性。波长在500~800 nm,癌变细胞的散射光强明显高于正常细胞所对应的散射光强;正常细胞所对应的光谱曲线中,800~1000 nm范围内有规则的强度变化,癌细胞没有出现这种干涉;说明本装置适用于细胞的癌变检测,而且实验结果与现有理论预测结果一致。理解不同病理阶段的细胞光散射特性有助于为在体实时检测早期癌变组织提供有利的光学手段。
In order to experimentally analyze the difference between the microscopic backscattering spectra of human normal gastric epithelial cells and cancerous cells and provide experimental evidence for clinical medical detection, based on the optical fiber confocal microscopy and combined with the theory of cell scattering, a Set based on optical confocal cell detection microspectroscopy device, can simultaneously obtain cell microscopy and microscopic medical information. The apparatus was used to measure the adherent human normal and cancerous gastric epithelial cells to obtain the cellular level of the microscopic spectral characteristics. The wavelength of 500 ~ 800 nm, cancerous cells scattered light intensity was significantly higher than normal cells corresponding to the scattered light intensity; normal cells corresponding to the spectral curve, 800 ~ 1000 nm within the scope of the intensity of the regular changes in cancer cells did not appear This interference; indicating that the device is suitable for cancer detection of cells, and the experimental results consistent with the existing theoretical predictions. Understanding the light scattering characteristics of cells at different pathological stages helps to provide an advantageous optical approach for detecting early cancerous tissues in vivo in real time.