This study dealt with the distribution characteristics of soil organic carbon (SOC) and the variation of stable carbon isotopic composition (δ13C values) with depth in six soil profiles, including two soil types and three vegetation forms in the karst areas of Southwest China. The δ13C values of plant-dominant species, leaf litter and soils were measured using the sealed-tube high-temperature combustion method. Soil organic carbon contents of the limestone soil profiles are all above 11.4 g/kg, with the highest value of 71.1 g/kg in the surface soil. However, the contents vary between 2.9 g/kg and 46.0 g/kg in three yellow soil profiles. The difference between the maximum and minimum δ13C values of soil organic matter (SOM) changes from 2.2‰ to 2.9‰ for the three yellow soil profiles. But it changes from 0.8‰ to 1.6‰ for the limestone soil profiles. The contrast research indicated that there existed significant difference in vertical patterns of organic carbon and δ13C values of SOM between yellow soil and limestone soil. This difference may reflect site-specific factors, such as soil type, vegetation form, soil pH value, and clay content, etc., which control the contents of different organic components comprising SOM and soil carbon turnover rates in the profiles. The vertical variation patterns of stable carbon isotope in SOM have a distinct regional character in the karst areas. This study dealt with the distribution characteristics of soil organic carbon (SOC) and the variation of stable carbon isotopic composition (δ13C values) with depth in six soil profiles, including two soil types and three vegetation forms in the karst areas of Southwest China. δ13C values ​​of plant-dominant species, leaf litter and soils were measured using the sealed-tube high-temperature combustion method. Soil organic carbon contents of the limestone soil profiles are all above 11.4 g / kg, with the highest value of 71.1 g / kg in the surface soil. However, the contents vary between 2.9 g / kg and 46.0 g / kg in three yellow soil profiles. The difference between the maximum and minimum δ13C values ​​of soil organic matter (SOM) changes from 2.2 ‰ to 2.9 ‰ for the three yellow soil profiles. But it changes from 0.8 ‰ to 1.6 ‰ for the limestone soil profiles. The contrast research indicates that there exists significant difference in vertical patterns of organic carbon and δ13C valu es of SOM between yellow soil and limestone soil. This difference may reflect site-specific factors, such as soil type, vegetation form, soil pH value, and clay content, etc., which control the contents of different organic components including SOM and soil carbon turnover rates in the profiles. The vertical variation patterns of stable carbon isotope in SOM have a distinct regional character in the karst areas.