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目的观察补阳还五汤对缺血性脑卒后遗症大鼠神经增殖作用的影响。方法采用机械开颅电凝法阻断大脑中动脉24h后,用随机电脉冲刺激仪每天刺激2h,连续刺激20 d,造成大鼠缺血性脑卒后遗症模型。治疗组在造模结束后灌服补阳还五汤水煎液,连续给药15 d。给药结束后处死大鼠,并取出大脑,处死前均腹腔注射5-溴脱氧核苷尿嘧啶 (BrdU),用免疫组织化学方法检测大鼠脑组织中BrdU的表达。结果与模型组相比,补阳还五汤治疗组BrdU阳性细胞数量显著增多。结论补阳还五汤对缺血性脑卒后遗症大鼠神经增殖具有促进作用。
Objective To observe the effect of Buyang Huanwu decoction on nerve proliferation in rats with sequelae of ischemic stroke. Methods After 24 hours of blocking the middle cerebral artery by mechanical craniotomy, the rats were stimulated for 2 hours per day with a random electric pulse stimulator and stimulated continuously for 20 days. This model resulted in ischemic stroke sequelae. The treatment group was fed with decoction of Buyang Huanwu Decoction at the end of the model and continued for 15 days. After the administration, the rats were sacrificed, and the brains were removed. The bromodeoxyuridine (BrdU) was injected intraperitoneally before being sacrificed. The expression of BrdU in the rat brain was detected by immunohistochemistry. Results Compared with the model group, the number of BrdU-positive cells in the treatment group of Buyang Huanwu Decoction significantly increased. Conclusion Buyang Huanwu decoction can promote the neural proliferation of ischemic stroke sequelae.