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目的探讨一种新型HLA衍生肽(RDP1258)对大鼠脾细胞增殖的体内免疫抑制作用及其机制。方法人工固相合成RDP1258,用3H-TdR掺入法观察其对体内大鼠脾细胞增殖反应的影响。采用酶化学法及免疫组化染色方法,观察其对体内脾细胞血红素氧合酶-1(heme oxygenase-1,HO-1)活性的影响。结果3H-TdR掺入法检测表明,RDP1258可显著抑制脾淋巴细胞转化及MLR的增殖反应;但能够明显提高体内HO-1的活性,并增强其表达。结论RDP1258体内应用能够显著抑制大鼠脾细胞由丝裂原或同种抗原引起的增殖反应,这种作用可能是通过影响HO-1的活性而实现。
Objective To investigate the immunosuppressive effect of a novel HLA-derived peptide (RDP1258) on proliferation of rat splenocytes in vitro and its mechanism. Methods Artificial solid phase synthesis of RDP1258, 3H-TdR incorporation method to observe the proliferation of rat spleen cell response. The effect of heme oxygenase-1 (HO-1) on the spleen cells in vivo was observed by enzymatic chemistry and immunohistochemical staining. Results 3H-TdR incorporation assay showed that RDP1258 could significantly inhibit the proliferation of splenic lymphocytes and MLR, but significantly increased the activity of HO-1 and enhanced the expression of HO-1 in vivo. Conclusion The in vivo application of RDP1258 can significantly inhibit the proliferative response induced by mitogen or alloantigen in rat spleen cells. This effect may be achieved by affecting the activity of HO-1.