Objective:The aim of this study was to detect apoptosis rates of Her-2 overexpression breast cancer cells,which were administrated with vitamin E succinate(VES) combined with paclitaxel at different dosages,or administrated alone;to discuss the mechanism of their actions.Methods:Using immunohistochemical method to detect Her-2 expression of MDAMB-453 cells.Using TUNEL assay to detect apoptosis rates of MDA-MB-453 cells,with the concentrations at 10,20 mg/L of VES and 50,100 nmol/L of paclitaxel,and also combined together for 24 or 48 h.Then compared apoptosis action of various combinations.Results:The expression rate of 95% Her-2 was interval(63.32%,69.60%);VES and paclitaxel both induced apoptosis of MDA-MB-453 cells,and it is dose to time dependence.It was strongest in apoptosis at 10 mg/L VES and 100 nmol/L paclitaxel in MDA-MB-453 cells 48 h later.Conclusion:VES and paclitaxel both induced apoptosis of MDA-MB-453 cells.It is stronger when the two drugs are administrated together.The mechanism is probably related to reduction of bcl-2 expression,so as to be more sensitive to paclitaxel.Synergistic effect is also possible for the two drugs influence tumor cells in different growing phases. Objective: The aim of this study was to detect apoptosis rates of Her-2 overexpression breast cancer cells, which were administered with vitamin E succinate (VES) combined with paclitaxel at different dosages, or administrated alone; to discuss the mechanism of their actions. Methods: Using immunohistochemical method to detect Her-2 expression of MDAMB-453 cells. Using TUNEL assay to detect apoptosis rates of MDA-MB-453 cells with the concentrations at 10, 20 mg / L of VES and 50, 100 nmol / L of paclitaxel, and also combined for 24 or 48 h. Comparison of the apoptosis action of various combinations. Results: The expression rate of 95% Her-2 was interval (63.32%, 69.60%); VES and paclitaxel both induced apoptosis of MDA- MB-453 cells, and it is dose to time dependence. It was strongest in apoptosis at 10 mg / L VES and 100 nmol / L paclitaxel in MDA-MB-453 cells for 48 h later. Confluence: VES and paclitaxel both induced apoptosis of MDA-MB-453 cells .It is stronger when the two drugs are administrated together.Th e mechanism is probably related to reduction of bcl-2 expression, so as to be more sensitive to paclitaxel.Synergistic effect is also possible for the two drugs affecting tumor cells in different growing phases.