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[目的]研究不同给药途径的CVP抗CCl4肝损伤的作用及机制。[方法]昆明种4月龄雌性小鼠40只,分对照组、模型组、CVP灌胃和饲料中加CVP保护组。饲养8d,末次灌胃2h,正常对组腹腔注射调和油溶液,其余各组腹腔注射0.15%CCl4调和油溶液(10ml/kg体重),18h后眼球取血分离血清,取出肝脏称重计算肝体指数,制备10%的肝匀浆,测定血清中ALT和AST的活性,检测肝匀浆中SOD、NOS的活性和MDA、GSH和NO的含量。[结果]不同给药途径的CVP保护组与模型组比较,可降低肝体指数(P﹤0.05)和MDA的含量(P﹤0.001),降低血清中AST活性(P﹤0.05),NOS活性及NO的含量(P﹤0.01),提高SOD的活性和GSH的含量(P﹤0.01)。[结论]不同给药途径的CVP对CCl4肝损伤均有保护作用。
[Objective] To investigate the effect and mechanism of CVP on anti-CCl4 liver injury in different route of administration. [Method] Forty-four-month-old female Kunming mice were divided into control group, model group, CVP group and CVP group. The rats in each group were injected intraperitoneally with 0.15% CCl4 oil (10ml / kg body weight) intraperitoneally. After 18 hours, the eyeballs were taken out of the blood to separate the serum, and the liver was taken out and weighed to calculate the liver mass Index, prepare 10% of liver homogenate, measure serum ALT and AST activity, detect liver homogenate SOD, NOS activity and MDA, GSH and NO content. [Results] Compared with the model group, the hepatic body mass index (P <0.05) and the content of MDA (P <0.001) and the activities of AST and AST NO content (P <0.01), and increased SOD activity and GSH content (P <0.01). [Conclusion] CVP with different route of administration has a protective effect on CCl4 liver injury.