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用限制性片段长度多态性(Restriction fragment length polymorphism,RFLP)方法研究了在农业废物堆肥一次发酵和二次发酵期间添加黄孢原毛平革菌(P.chrysosporium)对微生物多样性的影响。结果表明,在不同发酵期接种P.chrysosporium对堆肥进程的影响不同。3种典型的限制性内切酶AluⅠ、HaeⅢ和TaqⅠ在分析堆肥细菌微生物多样性的灵敏性上,HaeⅢ效果最好,AluⅠ次之,TaqⅠ则并不很适用于分析堆肥细菌微生物多样性。接种黄孢对堆肥细菌群落的影响非常显著且迅速,二次接种可起到巩固一次接种效果的作用。
Restriction fragment length polymorphism (RFLP) method was used to study the effect of adding P. chrysosporium on microbial diversity during primary and secondary fermentation of agricultural waste composting. The results showed that the effects of P. chrysosporium on the composting process were different at different fermentation stages. The three kinds of typical restriction enzymes AluⅠ, HaeⅢ and TaqⅠhad the best effect in analyzing the microbial diversity of compost bacteria, followed by AluⅠ, while TaqⅠwas not suitable for the analysis of microbial diversity of compost bacteria. Inoculation of yellow spores on the compost bacterial community impact is very significant and rapid, secondary vaccination can play a role in consolidating a vaccination effect.