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目的 评价胶体染料试纸条法诊断湄公血吸虫病的应用价值。方法 制备以日本血吸虫虫卵可溶性抗原为抗原的胶体染料试纸条试剂盒 (DDIA kit)。采用该试剂盒检测来自柬埔寨湄公血吸虫病流行区的 34例粪检阳性者和 170例粪检阴性者 ,近年来经吡喹酮治疗后不同年份的人群12 2例 ,以及 114例来自非血吸虫病流行区的健康人 ;同时对来自老挝湄公血吸虫病流行区的 70例粪检阳性者和 6 0例猫后睾吸虫感染者进行检测。对其中部分样本还采用日本血吸虫 SEA- EL ISA进行对照检测。结果 DDIA试剂盒检测湄公血吸虫病的敏感性为 97.1% (33/ 34,柬埔寨 )和98.6 % (6 9/ 70 ,老挝 ) ,与 EL ISA的检测结果相一致。对非流行区健康人的特异性为 10 0 .0 %。结论 日本血吸虫 DDIA试剂盒具有快速、简便、不需任何仪器设备等优点 ,同样适用于对湄公血吸虫病的现场大规模筛查
Objective To evaluate the value of colloidal dye strip test in the diagnosis of Mekong schistosomiasis. Methods The colloidal dye test strip kit (DDIA kit) was constructed with the antigen of Schistosoma japonicum egg soluble as antigen. The kit was used to detect 34 cases of positive stool tests and 170 cases of negative stool samples from endemic areas of Mekong schistosomiasis in Cambodia. In recent years, 122 cases of different ages after praziquantel treatment and 114 cases of non-schistosomiasis Meanwhile, 70 cases of positive stool samples and 60 cases of post-cynomolgus sinensis infection from the prevalence area of Mekong’s schistosomiasis in Laos were detected. Some of the samples were also used for the control test of Schistosoma japonicum SEA-EL ISA. Results The sensitivity of the DDIA kit for detection of Mekong schistosomiasis was 97.1% (33/34, Cambodia) and 98.6% (69/70, Laos), consistent with the EL ISA test. The specificity for healthy people in non-endemic areas was 100.0%. Conclusion The Schistosoma japonicum DDIA kit has the advantages of fast, simple, without any equipment and other advantages, the same applies to large-scale on-site screening of Mekong schistosomiasis