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目的 鉴定人玻璃体膜及视网膜前膜的细胞成分。 方法 对增生性玻璃体视网膜病变（ＰＶＲ）１２ 例和外伤性ＰＶＲ ８ 例患者经玻璃体手术取出的增生膜标本，用鼠抗人角蛋白、波形蛋白、神经胶质纤维酸性蛋白（ ＧＦＡＰ） 和ＣＤ１４等４ 种单抗做免疫组织化学ＡＢＣ 法染色并观察。 结果 １２ 例ＰＶＲ 膜抗角蛋白染色均为阳性，６ 例抗ＧＦＡＰ 阳性，１０ 例抗ＣＤ１４ 阳性；８ 例外伤性ＰＶＲ 膜２ 例抗角蛋白染色阳性，７ 例抗ＧＦＡＰ 阳性，３ 例抗ＣＤ１４ 阳性。未见抗波形蛋白阳性染色。 结论 视网膜色素上皮细胞和胶质细胞是ＰＶＲ 增生膜的主要细胞成分，但外伤性ＰＶＲ 膜中胶质细胞更多见。巨噬细胞参与ＰＶＲ 膜的形成
Objective To identify the cell components of human vitreous and retinal membranes. Methods Proliferative membrane specimens from 12 patients with proliferative vitreoretinopathy (PVR) and 8 patients with traumatic PVR who were treated by vitreous opacification were examined with anti-human keratin, vimentin, glial fibrillary acidic protein (GFAP) and CD14 Four monoclonal antibodies were immunohistochemically stained and observed by ABC method. Results 12 cases of PVR membrane were positive for keratin staining, 6 cases were anti-GFAP positive and 10 cases anti-CD14 positive. 8 cases of traumatic PVR membrane were positive for anti-keratin staining, 7 cases were anti-GFAP positive and 3 cases anti-CD14 positive . No anti-vimentin positive staining. Conclusion Retinal pigment epithelial cells and glial cells are the main cellular components of PVR hyperplasia, but glial cells are more common in traumatic PVR. Macrophages participate in the formation of PVR membranes