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对四羧基铝酞菁(AlC_4Pc)在大范围酸度介质中的荧光光谱行为进行了考察和分析,探索其作为pH测定比率型红色荧光探针的可行性。AlC_4Pc长波区域(红色)的特征荧光发射随着介质pH的增加而升高,对其荧光激发光谱进行深入分析,获得了重要的发现:AlC_4Pc在长波激发带(Q带)的荧光强度比率(激发波长分别为615nm和622nm)与介质的pH在大范围内(5.0~12.1)呈线性正相关。以参比法测定了AlC_4Pc的相对荧光量子产率,结果表明AlC_4Pc荧光量子效率较高,具有分析应用价值。结合吸收光谱研究探讨了AlC_4Pc对pH的长程响应机理,认为AlC_4Pc对pH的长程响应是由于AlC_4Pc的多步电离与分子间缔合综合作用而引起。上述研究提示AlC_4Pc有望成为新型比率型pH红色荧光探针。将该探针和建立的方法应用于多种实际样品的荧光比率测定,取得了较满意的结果。
Fluorescence spectra of tetracarboxyaluminum phthalocyanine (AlC_4Pc) in a wide range of acidity media were investigated and analyzed, and its feasibility as a pH-sensitive red fluorescent probe was explored. Fluorescence emission of AlC_4Pc in long wavelength region (red) increased with the increase of pH, and further analysis of its fluorescence excitation spectra revealed important discoveries: the fluorescence intensity ratio of AlC_4Pc in the long-wave excitation band (Q band) Wavelength 615nm and 622nm respectively) and the pH of the medium in a wide range (5.0 ~ 12.1) showed a linear positive correlation. The relative fluorescence quantum yield of AlC_4Pc was determined by the reference method. The results show that the fluorescence quantum efficiency of AlC_4Pc is high, which has the value of analysis and application. The long-range response mechanism of AlC_4Pc to pH was discussed based on absorption spectroscopy. The long-range response of AlC_4Pc to pH was thought to be caused by the combined effect of multi-step ionization and intermolecular association of AlC_4Pc. These studies suggest that AlC_4Pc is expected to become a new type of pH red fluorescent probe. The probe and the established method were applied to a variety of real samples of fluorescence ratio determination, and achieved satisfactory results.