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目的探讨不同孕期铅暴露状态下,大鼠血栓调节蛋白(thrombomodulin,TM)的变化以及在胚胎发育中的作用。方法 108只大鼠随机分为A,B,C,D 4组,A组为对照组,B,C,D为实验组。0.025%醋酸铅对实验组大鼠染铅。原子吸收光谱法测定孕鼠血铅水平。ELISA法测定孕鼠血及胎盘TM表达。结果实验组孕鼠血铅水平、血浆TM和胎盘TM水平均显著高于对照组(P<0.01),而实验组子鼠体重显著低于对照组(P<0.01)。血铅水平与血浆TM和胎盘TM之间以及血浆TM与胎盘TM水平之间呈显著相关,r值分别为:0.9063;0.9697和0.9702。结论孕期不同阶段的铅暴露对TM表达的影响不同,TM可能在维持胎盘组织凝血-抗凝平衡和胚胎发育中发挥重要作用。
Objective To investigate the changes of rat thrombomodulin (TM) and its role in embryogenesis during lead exposure at different stages of pregnancy. Methods 108 rats were randomly divided into A, B, C, D 4 groups, A group as control group, B, C, D as experimental group. Lead in the test group was 0.025% lead acetate. Determination of Blood Lead Level in Pregnant Rats by Atomic Absorption Spectrometry. Measurement of serum and placenta TM expression in pregnant rats by ELISA. Results The levels of blood lead, plasma TM and placental TM in experimental group were significantly higher than those in control group (P <0.01), while those in experimental group were significantly lower than those in control group (P <0.01). There was a significant correlation between blood lead levels and plasma TM and placenta TM as well as plasma TM and placental TM levels, with r values of 0.9063, 0.9697 and 0.9702, respectively. Conclusion The effects of lead exposure at different stages of pregnancy on TM expression are different. TM may play an important role in maintaining the balance of coagulation-anticoagulation and embryo development in placenta.