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目的:观察肥胖乳腺癌患者乳腺癌组织中磷酯酰肌醇3激酶(PI3K)/Akt信号通路的活性。方法:选取45例肥胖乳腺癌患者(肥胖组)与37例正常体质量乳腺癌患者(对照组)的乳腺癌组织标本,分别用RT-PCR法检测两组乳腺癌组织中PI3K mRNA的表达,及免疫组化法检测PI3K和磷酸化Akt(p-Akt)蛋白的表达。结果:RT-PCR结果显示,肥胖组乳腺癌组织中PI3K mRNA表达较对照组明显增高(P<0.05);免疫组化结果显示,肥胖组PI3K和p-Akt蛋白表达均明显升高(均P<0.05),且PI3K与p-Akt蛋白表达水平在两种组织中均呈正相关(r=0.76,r=0.81,均P<0.05)。结论:肥胖乳腺癌患者癌组织中PI3K/Akt信号通路活性明显高于非肥胖患者,故推测这可能是导致肥胖者乳腺癌风险增高的原因之一。
Objective: To observe the activity of phosphatidylinositol 3 - kinase (PI3K) / Akt signaling pathway in breast cancer tissues from patients with obesity. Methods: Forty-five patients with obese breast cancer (obese group) and 37 normal breast cancer patients (control group) were selected for breast cancer. The expression of PI3K mRNA in breast cancer tissues was detected by RT-PCR. Immunohistochemistry was used to detect the expression of PI3K and phosphorylated Akt (p-Akt) protein. Results: RT-PCR results showed that the expression of PI3K mRNA in obese group was significantly higher than that in control group (P <0.05). The results of immunohistochemistry showed that the expressions of PI3K and p-Akt in obese group were significantly increased <0.05). There was a positive correlation between PI3K and p-Akt protein expression in both tissues (r = 0.76, r = 0.81, all P <0.05). Conclusion: The PI3K / Akt signaling pathway in obese breast cancer patients was significantly higher than that in non-obese patients, suggesting that this may be one of the reasons for the increased risk of breast cancer in obese patients.