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采用ＳｅｐｈａｄｅｘＧ５０、ＣＭ－Ｃｅｌｕｌｏｓｅ３２柱层析，从中华眼镜蛇中分离出神经生长因子（ＮｅｒｖｅＧｒｏｗｔｈＦａｃｔｅｒ，ＮＧＦ）。经ＳＤＳ－聚丙烯酰胺凝胶电泳及Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ法证明所得到的ＮＧＦ为单一组分，相对分子质量约为１．３×１０４．经凝胶等电聚焦电泳测得ＮＧＦ等电点ＰＩ约为７．０左右．经ＨＰＬＣ及电泳图像分析系统测得纯度为９８％．此ＮＧＦ经８ｄ鸡胚背根神经节体外培养证明，具有促进神经纤维生长的活性．将所纯化的ＮＧＦ免疫大白兔获得抗血清，经ｐｒｏｔｅｉｎＡ－Ｓｅｐｈａｒ－ｓｏｅＣＬ－４Ｂ亲和层析柱纯化获得了较为单一的抗ＮＧＦＩｇＧ
Nerve Growth Factor (NGF) was isolated from Chinese cobra by SephadexG50 and CM-Celulose32 column chromatography. SDS-polyacrylamide gel electrophoresis and Western blotting showed that the resulting NGF was a single component with a relative molecular mass of about 1.3 × 104. Gel isoelectric focusing electrophoresis measured NGF isoelectric point PI is about 7.0. The purity was 98% by HPLC and electrophoresis image analysis system. This NGF by 8d chick embryo dorsal root ganglia in vitro culture proved that with the promotion of nerve fiber growth activity. The purified NGF-immunized rabbits were obtained antiserum, purified by proteinA-Sephar-soeCL-4B affinity chromatography to obtain a single anti-NGFIgG