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为了利用废弃油脂制备生物表面活性剂,用嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)WO-S14对不同植物油脂进行发酵,并对产物进行系统分析。通过发酵试验与性能分析,发现芝麻油的发酵产物具有优良的表面性能,对煤油的乳化率E24为75.22%,使水的表面张力降低至27.90 m N/m,且在较广的环境条件范围内具有良好稳定性。采用柱层析方法获得分离纯化的表面活性剂产物,通过薄层层析(TLC)、红外光谱(IR)、气质联用(GC-MS)、电喷雾质谱(ESI-MS)和串联质谱(MS/MS)等手段分析了产物的化学组成和结构,结果表明以芝麻油生物合成的表面活性剂主要是一种具有环状结构的脂肽,分子量为814,由含有15个碳原子的β-羟基脂肪酸和含有6个氨基酸分子的短肽组成,肽链中氨基酸连接顺序为:-Met-Glu-Ala-Ser-Ala-Val-,是一种新型脂肽类生物表面活性剂。
In order to prepare biosurfactants from waste grease, different vegetable oils were fermented with Stenotrophomonas maltophilia WO-S14 and the products were subjected to a systematic analysis. Through the fermentation test and performance analysis, it was found that the fermentation product of sesame oil has excellent surface properties, the emulsification rate of kerosene E24 is 75.22%, the surface tension of water is reduced to 27.90 m N / m, and in a wide range of environmental conditions Has good stability. The purified and purified surfactant products were obtained by column chromatography and identified by TLC, IR, GC-MS, ESI-MS and tandem mass spectrometry MS / MS). The results showed that the biosynthesis surfactant with sesame oil was mainly a lipopeptide with a ring structure with a molecular weight of 814, which consisted of β- Hydroxy fatty acid and short peptide containing 6 amino acids. The sequence of the amino acid in the peptide chain is: Met-Glu-Ala-Ser-Ala-Val-, which is a novel lipopeptide biosurfactant.