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为了明确二斑叶螨Tetranychus urticae Koch对阿维菌素的抗性水平,采用玻片浸渍法测定了北京4个地区二斑叶螨雌成螨对阿维菌素的抗药性,建立了特异性等位基因PCR(PASA)方法,并检测了二斑叶螨谷氨酸门控氯离子通道(GluCl)基因片段上G323D的突变频率。结果显示,北京昌平、海淀、密云和怀柔4个田间二斑叶螨种群对阿维菌素均达极高抗性水平,其中昌平种群抗性最高,LC50值为448.93 mg/L,抗性倍数为4988.11倍。室内敏感种群未见抗性个体,昌平和密云种群G323D等位基因突变频率为100.00%,怀柔和海淀种群分别为86.25%和90.00%。北京4个地区二斑叶螨种群对阿维菌素达极高抗性水平,抗性基因的突变频率也很高,表明阿维菌素不适宜用来防治这些地区的二斑叶螨种群。
In order to clarify the resistance level of Tetranychus urticae Koch to avermectin, the susceptibility to abamectin in female Tetranychus urticae Koch was determined by glass slide dipping method in four areas of Beijing, and the specificity was established Allele PCR (PASA) method and the mutation frequency of G323D on GluCl gene fragment of two-spotted spider mite was detected. The results showed that the two populations of Tetranychus urticae in four fields of Changping, Haidian, Miyun and Huairou in Beijing were all highly resistant to avermectin, of which Changping population had the highest resistance with a LC50 value of 448.93 mg / L and a multiple of resistance 4988.11 times. The indoor susceptible population showed no resistance. The frequency of G323D allele mutation was 100.00% in Changping and Miyun populations, and 86.25% and 90.00% in Huairou and Haidian populations, respectively. The population of Tetranychus urticae in four areas of Beijing reached extremely high levels of resistance to abamectin, and the mutation frequency of resistance genes was also high, indicating that abamectin is not suitable for the control of Tetranychus urticae in these areas.