论文部分内容阅读
目的:调查2种抗菌制剂外排泵基因(mdfA、qacE△1)和7种可移动遗传元件(traA、trbC、IS1133、ISEcp1、tnpU、tnp513、qacE△1)在多耐药肺炎克雷伯菌中的存在和变异情况。方法:收集绍兴市人民医院2007年10月-2009年6月住院患者标本中分离的多耐药肺炎克雷伯菌共20株,采用聚合酶链反应(PCR)及序列分析的方法分析上述8种基因。结果:20株多耐药肺炎克雷伯菌共检出7种基因,mdfA、traA、trbC、ISEcp1、tnpU、tnp513、qacE△1基因的阳性株数分别为18株(90.0%)、2株(10.0)、16株(80.0%)、14株(70.0%)、8株(40.0%)、11株(55.0%)、18株(90.0%),只有IS1133基因未能检出。且这7种阳性基因以7种阳性模式存在,其中(mdfA+trbC+ISEcp1+qacE△1)模式的检出率最高,共6株(30.0%)。结论:抗菌制剂外排泵基因和可移动遗传元件阳性率很高,这是导致本组菌株呈多耐药的一个重要原因。
Objective: To investigate the effects of two kinds of antimicrobial efflux pump genes (mdfA, qacE △ 1) and seven kinds of mobile genetic elements (traA, trbC, IS1133, ISEcp1, tnpU, tnp513 and qacE △ 1) The presence and variation in bacteria. Methods: A total of 20 multidrug-resistant Klebsiella pneumoniae isolates from hospitalized patients in Shaoxing People’s Hospital from October 2007 to June 2009 were collected and analyzed by polymerase chain reaction (PCR) and sequence analysis Genes. RESULTS: Seven strains were detected in 20 multidrug-resistant Klebsiella pneumoniae strains. The number of positive mdfA, traA, trbC, ISEcp1, tnpU, tnp513 and qacE △ 1 genes was 18 (90.0%) and 2 10.0), 16 isolates (80.0%), 14 isolates (70.0%), 8 isolates (40.0%), 11 isolates (55.0%) and 18 isolates (90.0%). The seven positive genes were present in seven positive modes, of which the highest detection rate was (mdfA + trbC + ISEcp1 + qacE △ 1), with a total of 6 strains (30.0%). Conclusion: The positive rate of efflux pump genes and mobile genetic elements of antimicrobial agents is very high, which is one of the most important causes of multidrug resistance in this group of strains.