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以口腔脱落细胞ＤＮＡ为模板，采用扩增不应突变系统（ＡＲＭＳ），设计了等位基因４个特异性寡核苷酸引物和一个公共引物，分析了７４例个体ＡｐｏＥ３个常见共显性等位基因∈２、∈３和∈４。这一系统扩增出１８１ｂｐ和３１９ｂｐ两种ＡｐｏＥ基因顺序，当携某一等位基因的模板ＤＮＡ与相应的等位基因特异性寡核着酸引物及公共引物反应时，扩增出相应的基因顺序。省略了限制性核酸内切酶的消化或等位基因特异性寡核苷酸探针的杂交。方法简单、可靠、非放射性。
Using exfoliated cell DNA as a template, four alleles-specific oligonucleotide primers and a common primer were designed by using ARMS, and the common codominance of ApoE3 was analyzed in 74 individuals Bit genes ∈2, ∈3 and ∈4. This system amplified two ApoE gene sequences of 181 bp and 319 bp. When the template DNA carrying one allele reacted with the corresponding allele-specific oligonucleotide primer and common primer, the corresponding gene was amplified order. Digestion of restriction endonucleases or hybridization of allele-specific oligonucleotide probes is omitted. Method is simple, reliable, non-radioactive.