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建立测定密骨胶囊中淫羊藿苷含量的方法。样品用甲醇超声提取 30 m in,在 C1 8柱上以甲醇 -水 -醋酸 (45∶5 0∶ 5 )为流动相 ,在波长 2 70 nm处检测。淫羊藿苷含量测定的线性范围为 0 .0 5 0 9~ 0 .8144μg,线性回归方程为Y=1479.437+4 5 5 0 5 1.2 13X,r=0 .9999,平均回收率为 98.95 % ,RSD为 2 .44 %。该法简便、准确、重现性好 ,可作为密骨胶囊质量控制的方法。
To establish a method for the determination of icariin content in Migu capsules. The sample was extracted with methanol for 30 min in an ultrasonic bath and methanol-water-acetic acid (45:50:0:5) was used as the mobile phase on a C18 column at a wavelength of 270 nm. The linear range of the determination of icariin content was 0.05 to 0.81444 μg, the linear regression equation was Y=1479.437+4 5 5 5 5 1.2 13X, r=0.9999, and the average recovery was 98.95 %. RSD is 2.44%. The method is simple, accurate, and reproducible, and can be used as a method for quality control of the TCM capsule.