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目的:研究外源性脆性组氨酸三联体(FHIT)基因的表达对乳腺癌MDA-MB-436细胞增殖的影响并探讨其机制。方法:通过脂质体将外源性FHIT基因的真核表达质粒PEGFP-FHIT和空载体分别转染FHIT表达缺失的乳腺癌细胞MDA-MB-436。MTT法分析细胞增殖,流式细胞术观察细胞的凋亡,蛋白质印迹法检测外源性FHIT基因及凋亡相关基因Caspase-3、-8、-9的蛋白表达。结果:PEGFP-FHIT组细胞的生长抑制率和凋亡率明显高于空载体组和对照组,PEGFP-FHIT组细胞Caspase-3、-9活性片段表达上调。结论:外源性FHIT基因表达能抑制MDA-MB-436细胞增殖,其机制可能是通过激活Caspase途径从而诱导细胞凋亡。
Objective: To investigate the effect of exogenous FHIT gene on the proliferation of breast cancer MDA-MB-436 cells and to explore its mechanism. Methods: Eukaryotic expression plasmid PEGFP-FHIT of exogenous FHIT gene and empty vector were transfected into MDA-MB-436 breast cancer cells with FHIT deletion. Cell proliferation was analyzed by MTT assay. Apoptosis was observed by flow cytometry. Protein expression of exogenous FHIT gene and apoptosis related gene Caspase-3, -8, -9 were detected by Western blotting. Results: The growth inhibition rate and apoptosis rate of PEGFP-FHIT group were significantly higher than that of empty vector group and control group. The expression of Caspase-3 and -9 in PEGFP-FHIT group was up-regulated. Conclusion: Exogenous FHIT gene expression can inhibit the proliferation of MDA-MB-436 cells. The mechanism may be through the activation of Caspase pathway to induce apoptosis.