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用帝国、自由、富士、嘎拉、金冠、乔纳金、Ｊｏｎａｓｔｙ、红乔纳金、解放、皇家嘎拉１０个苹果品种的试管苗，切取叶块作离体培养试验。枝条的再生程序是：０．５ｍ￣２大小的叶块，在再生培养基上，黑暗下培养两周时间，首先从叶切块基部的中脉处形成愈伤组织，然后是侧脉和其它部分陆续形成愈伤组织；经两周黑暗处理后，再在光照下培养约两周时间，就能从愈伤组织上开始生出枝条。再生能力的大小受品种基因型所左右，Ｊｏｎａｓｔｙ是一个高度亲合的三倍体品种，再生能力最强。其次是乔纳金、自由和帝国。最好的再生培养基是ＭＳＳａｌｔ＋４０ｍｇ／Ｌ、ＬＳＶｉｔａｍｉｎ＋１ｍｇ／Ｌ、ＴＤＺ＋０．５ｍｇ／Ｌ、ＩＡＡ。
In vitro experiments were carried out on the test-tube seedlings of 10 apple cultivars including Imperial, Free, Fuji, Gala, Golden Delicious, Jonathan, Jonasty, Red Jonathan, Liberation and Royal Gala. The shoot regeneration procedure is: 0.5m ~ 2 leaf size, regenerated medium, dark for two weeks, first from the midpoint of the leaf cut into the formation of callus at the base, then the lateral veins and other parts Callus gradually formed; after two weeks of darkness, and then cultured in light for about two weeks, we can start from the callus on the branches. The size of regenerative ability is influenced by the genotype of the breed. Jonasty is a highly compatible triploid breed with the strongest regenerative ability. Followed by Jonathan, freedom and empire. The best regeneration medium is MSSalt + 40mg / L, LSVitamin + 1mg / L, TDZ + 0.5mg / L, IAA.